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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chen, Jie Shi, Feng Zhang, Bin Zhu, Fan Cao, Weifeng Xu, Zhinan Xu, Guohua Cen, Peilin |
| Description | Author Affiliation: Chen J ( Institute of Bioengineering, Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, People's Republic of China.) |
| Abstract | Poly-gamma-glutamic acid (gamma-PGA) is a kind of water-soluble and biodegradable polymer made from D- and L-glutamic acid units, which are linked by amide bonds formed between alpha-amino and gamma-carboxylic acid groups. As a potential targeted biopolymer that can be refined from biomass directly, gamma-PGA has been increasingly applied to food, cosmetic, and pharmaceutical industries. In this work, a suitable nitrogen source was screened out for the high and cost-effective production of gamma-PGA in Bacillus subtilis ZJU-7. The effects of inoculation time and initial glucose concentration on the gamma-PGA production were investigated systematically in both shake flasks and a bench-top 15-l fermentor. Under the optimized culture conditions, a high gamma-PGA productivity (46.4 g/l) was obtained after 48 h cultivation at 37 degrees C. Finally, the large-scale fermentation of gamma-PGA production was successfully scaled up to a 100-l fermentor, with the highest gamma-PGA productivity for over 54.0 g/l. |
| File Format | HTM / HTML |
| ISSN | 02732289 |
| Issue Number | 2 |
| Volume Number | 160 |
| e-ISSN | 15590291 |
| Journal | Applied Biochemistry and Biotechnology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2010-01-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Discipline Biotechnology Bacillus Subtilis Metabolism Culture Techniques Methods Industrial Microbiology Polyglutamic Acid Analogs & Derivatives Chemistry Bioreactors Microbiology Culture Media Fermentation Glucose Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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