NDLI: Delayed growth of glioma by a polysaccharide from Aster tataricus involve upregulation of Bax/Bcl-2 ratio, activation of caspase-3/8/9, and downregulation of the Akt.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Du, Lei Mei, Hai-Feng Yin, Xue Xing, Yi-Qiao
Description	Country affiliation: China Author Affiliation: Du L ( Eye Center, Renmin Hospital of Wuhan University, Wuchang Jiefang Road 238, Wuhan, 430060, China.)
Abstract	In this study, a homogeneous polysaccharide (ATP-II), with a molecular weight of 3.4 × 10(4) Da, was successfully purified from Aster tataricus by DEAE-Sepharose CL-6B ion exchange and Sepharose CL-6B gel filtration chromatography. Monosaccharide component analysis indicated that ATP-II was composed of glucose, galactose, mannose, rhamnose, and arabinose in molar ratios of 2.1:5.2:2.1:1.0:1.2. We evaluated the anticancer efficacy and associated mechanisms of ATP-II on glioma C6 cells in vitro and in vivo. The results showed that treatment of C6 cells with ATP-II inhibited cell proliferation and this biological response came from induction of DAN damage and consequent inducing apoptosis. Likewise, oral ATP-II administration resulted in consistent regression of glioma tumors and induced apoptosis of transplanted tumor tissues by increasing the ratio of Bax/Bcl-2 and activation of caspase-3, caspase-8, and caspase-9 cascade. Importantly, the efficient downregulation of Akt, which is successfully detected in tumor tissues, is a unique contribution to retard the tumor growth by ATP-II. These data suggest that ATP-II may be a potential candidate for glioma treatment.
File Format	HTM / HTML
ISSN	10104283
Issue Number	3
Volume Number	35
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2014-03-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Antineoplastic Agents Pharmacology Aster Plant Chemistry Glioma Metabolism Phytotherapy Methods Plant Extracts Polysaccharides Animals Apoptosis Drug Effects Blotting, Western Caspases Cell Line, Tumor Cell Proliferation Down-regulation Enzyme Activation In Situ Nick-end Labeling Male Molecular Weight Isolation & Purification Proto-oncogene Proteins C-akt Proto-oncogene Proteins C-bcl-2 Rats Rats, Sprague-dawley Up-regulation Bcl-2-associated X Protein Journal Article
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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