NDLI: Identification of plant RAD52 homologs and characterization of the Arabidopsis thaliana RAD52-like genes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Samach, Aviva Melamed-Bessudo, Cathy Avivi-Ragolski, Naomi Pietrokovski, Shmuel Levy, Avraham A.
Description	Country affiliation: Israel Author Affiliation: Samach A ( Department of Plant Sciences, Weizman Institute of Science, Rehovot, Israel.)
Abstract	RADiation sensitive52 (RAD52) mediates RAD51 loading onto single-stranded DNA ends, thereby initiating homologous recombination and catalyzing DNA annealing. RAD52 is highly conserved among eukaryotes, including animals and fungi. This article reports that RAD52 homologs are present in all plants whose genomes have undergone extensive sequencing. Computational analyses suggest a very early RAD52 gene duplication, followed by later lineage-specific duplications, during the evolution of higher plants. Plant RAD52 proteins have high sequence similarity to the oligomerization and DNA binding N-terminal domain of RAD52 proteins. Remarkably, the two identified Arabidopsis thaliana RAD52 genes encode four open reading frames (ORFs) through differential splicing, each of which specifically localized to the nucleus, mitochondria, or chloroplast. The A. thaliana RAD52-1A ORF provided partial complementation to the yeast rad52 mutant. A. thaliana mutants and RNA interference lines defective in the expression of RAD52-1 or RAD52-2 showed reduced fertility, sensitivity to mitomycin C, and decreased levels of intrachromosomal recombination compared with the wild type. In summary, computational and experimental analyses provide clear evidence for the presence of functional RAD52 DNA-repair homologs in plants.
File Format	HTM / HTML
ISSN	10404651
e-ISSN	1531298X
DOI	10.1105/tpc.111.091744
Journal	THE PLANT CELL ONLINE
Issue Number	12
Volume Number	23
Language	English
Publisher	American Society of Plant Biologists
Publisher Date	2011-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Botany Arabidopsis Proteins Genetics Arabidopsis Rad51 Recombinase Rad52 Dna Repair And Recombination Protein Amino Acid Sequence Classification Drug Effects Metabolism Cell Nucleus Chloroplasts Computational Biology Evolution, Molecular Gene Duplication Gene Expression Regulation, Plant Genes, Plant Genetic Complementation Test Homologous Recombination Mitochondria Mitomycin Pharmacology Open Reading Frames Phylogeny Protein Isoforms Rna Interference Rna Splicing Saccharomyces Cerevisiae Saccharomyces Cerevisiae Proteins Sequence Alignment Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science

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