NDLI: Transposition of a rice Mutator-like element in the yeast Saccharomyces cerevisiae.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhao, Dongyan Ferguson, Ann Jiang, Ning
Description	Author Affiliation: Zhao D ( Department of Horticulture, Michigan State University, East Lansing, Michigan 48824.); Ferguson A ( Department of Horticulture, Michigan State University, East Lansing, Michigan 48824.); Jiang N ( Department of Horticulture, Michigan State University, East Lansing, Michigan 48824 jiangn@msu.edu.)
Abstract	Mutator-like transposable elements (MULEs) are widespread in plants and are well known for their high transposition activity as well as their ability to duplicate and amplify host gene fragments. Despite their abundance and importance, few active MULEs have been identified. In this study, we demonstrated that a rice (Oryza sativa) MULE, Os3378, is capable of excising and reinserting in yeast (Saccharomyces cerevisiae), suggesting that yeast harbors all the host factors for the transposition of MULEs. The transposition activity induced by the wild-type transposase is low but can be altered by modification of the transposase sequence, including deletion, fusion, and substitution. Particularly, fusion of a fluorescent protein to the transposase enhanced the transposition activity, representing another approach to manipulate transposases. Moreover, we identified a critical region in the transposase where the net charge of the amino acids seems to be important for activity. Finally, transposition efficiency is also influenced by the element and its flanking sequences (i.e., small elements are more competent than their large counterparts). Perfect target site duplication is favorable, but not required, for precise excision. In addition to the potential application in functional genomics, this study provides the foundation for further studies of the transposition mechanism of MULEs.
File Format	HTM / HTML
ISSN	10404651
e-ISSN	1531298X
DOI	10.1105/tpc.114.128488
Journal	THE PLANT CELL ONLINE
Issue Number	1
Volume Number	27
Language	English
Publisher	American Society of Plant Biologists
Publisher Date	2015-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Botany Oryza Sativa Metabolism Plant Proteins Saccharomyces Cerevisiae Dna Transposable Elements Genetics Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A rice Tc1/mariner-like element transposes in yeast.

A two-edged role for the transposable element Kiddo in the rice ubiquitin2 promoter.

Insertion preference of maize and rice miniature inverted repeat transposable elements as revealed by the analysis of nested elements.

Transposition of the rice miniature inverted repeat transposable element mPing in Arabidopsis thaliana.

Pack-Mutator-like transposable elements (Pack-MULEs) induce directional modification of genes through biased insertion and DNA acquisition.

DNA is structured as a linear 'jigsaw puzzle' in the genomes of Arabidopsis, rice, and budding yeast.

Transposition of a Rice Mutator-Like Element in the Yeast Saccharomyces cerevisiae

Efficient transposition of the youngest miniature inverted repeat transposable element family of yellow fever mosquito in yeast.

Evolution of the rice Xa21 disease resistance gene family.

Transposition of a rice Mutator-like element in the yeast Saccharomyces cerevisiae.

Similar Documents

A rice Tc1/mariner-like element transposes in yeast.

A two-edged role for the transposable element Kiddo in the rice ubiquitin2 promoter.

Insertion preference of maize and rice miniature inverted repeat transposable elements as revealed by the analysis of nested elements.

Transposition of the rice miniature inverted repeat transposable element mPing in Arabidopsis thaliana.

Pack-Mutator-like transposable elements (Pack-MULEs) induce directional modification of genes through biased insertion and DNA acquisition.

DNA is structured as a linear 'jigsaw puzzle' in the genomes of Arabidopsis, rice, and budding yeast.

Transposition of a Rice Mutator-Like Element in the Yeast Saccharomyces cerevisiae

Efficient transposition of the youngest miniature inverted repeat transposable element family of yellow fever mosquito in yeast.

Evolution of the rice Xa21 disease resistance gene family.

Transposition of a rice Mutator-like element in the yeast Saccharomyces cerevisiae.