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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sumitomo, Keiko Yamaguchi, Yoshinori Tatsuta, Kuniaki |
| Description | Country affiliation: Japan Author Affiliation: Sumitomo K ( Consolidated Research Institute for Advanced Science and Medical Care (ASMeW), Waseda University, Tokyo, Japan.) |
| Abstract | 1,2,5-Thiadiazole improved RNA separation with in-capillary denaturing polymer electrophoresis. 1,2,5-Thiadiazole was synthesized as an extraction solvent substituted for a halogenated solvent. While 1,2,5-thiadiazole was an excellent extraction solvent and an environmentally friendly solvent, we found that 1,2,5-thiadiazole was a strong hydrophobic compound for RNA and the RNA separation performance by in-capillary denaturing polymer electrophoresis was dramatically improved. We suggest 'in-capillary denaturing polymer electrophoresis' as an RNA separation that realizes the denaturing and separation simultaneously. RNA separation by the method required a strong denaturant, acetic acid, to cleave the intramolecular hydrogen. The running buffer containing acetic acid was of high conductivity and low pH, in which the condition introduced Joule heating and low sensitivity. While conventional denaturants, formaldehyde and urea, maintained small electric conductivity and neutral pH, these denaturants were too weak to achieve the RNA separation by in-capillary denaturing polymer electrophoresis. 1,2,5-Thiadiazole being a neutral molecule, both conductivity and buffer pH were able to be adjusted to a desirable strength for RNA separation. In this paper, we report that RNA separation by in-capillary denaturing polymer electrophoresis in neutral pH was achieved and the sensitivity for RNA separation was higher than that for RNA separation by in-capillary denaturing polymer electrophoresis with acetic acid. |
| File Format | HTM / HTML |
| ISSN | 16159306 |
| Issue Number | 20 |
| Volume Number | 34 |
| e-ISSN | 16159314 |
| Journal | Journal of Separation Science |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2011-10-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Electrophoresis, Capillary Methods Rna Isolation & Purification Thiadiazoles Chemistry Cellulose Instrumentation Hydrogen-ion Concentration Polymers Analysis Sensitivity And Specificity Solvents Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Filtration and Separation |
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