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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yamamoto, Sachio Watanabe, Yuki Nishida, Noriaki Suzuki, Shigeo |
| Description | Country affiliation: Japan Author Affiliation: Yamamoto S ( Faculty of Pharmacy, Kinki University, Kowakae, Higashi-Osaka, Osaka, Japan.) |
| Abstract | A method for the simultaneous concentration and separation of weak acids using an acidic polyacrylamide gel, fabricated in the microfluidic channel of a commercial poly(methyl methacrylate)-made microchip, is reported. This approach is based on simple photochemical copolymerization for the fabrication of a permselective preconcentrator. The intersection of the poly(methyl methacrylate)-made microchip was filled with a gel solution comprising acrylamide, N,N'-methylene-bis-acrylamide, and 2-acrylamidoglycolic acid, with riboflavin as a photocatalytic initiator. In situ polymerization, near the cross of the sample outlet channel, was performed by irradiation with an argon ion laser beam that is also used as the light source for fluorimetric detection. The electrokinetic properties, combined with electrostatic repulsion between sample components and the anionic groups on the polyacrylamide gel, enable the entrapment and concentration of weak acids at the interface of the cathodic side of the gel plug. This method displays concentration factors of up to 10(5) within 3 min. The effectiveness of the ionic preconcentrator was demonstrated by the sensitive analysis of fluorescein isothiocyanate-labeled amino acids. |
| File Format | HTM / HTML |
| ISSN | 16159306 |
| Issue Number | 20 |
| Volume Number | 34 |
| e-ISSN | 16159314 |
| Journal | Journal of Separation Science |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2011-10-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Amino Acids Chemistry Isolation & Purification Analytic Sample Preparation Methods Methods Electrophoresis, Microchip Acrylic Resins Instrumentation Fluorescein-5-isothiocyanate Fluorescent Dyes Photochemical Processes Polymerization Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Filtration and Separation |
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