NDLI: DJ-1 regulates mast cell activation and IgE-mediated allergic responses.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Do Kyun Kim, Hyuk Soon Kim, A-Ram Kim, Ji Hyung Kim, Bokyung Noh, Geunwoong Kim, Hyung Sik Beaven, Michael A. Kim, Young Mi Choi, Wahn Soo
Description	Author Affiliation: Kim DK ( Department of Immunology and Physiology, Functional Genomics Institute, College of Medicine, Konkuk University, Chungju, Korea.)
Abstract	BACKGROUND: DJ-1 is an antioxidant protein known to reduce levels of reactive oxygen species (ROS), but its presence or function in mast cells and allergic diseases is unknown. OBJECTIVES: We sought to determine the role and mechanism of DJ-1 in allergic responses in vitro and in vivo. METHODS: ROS and DJ-1 levels in serum or culture medium were measured with ELISA kits. The role of DJ-1 was evaluated in mast cell cultures and passive cutaneous anaphylaxis in normal or DJ-1 knockout (KO) mice. The mechanism of DJ-1 action was examined by using immunoblotting, immunoprecipitation, RT-PCR, and other molecular biological approaches. RESULTS: Patients with atopic dermatitis had increased levels of ROS and diminished levels of DJ-1. DJ-1 KO mice exhibited enhanced passive cutaneous anaphylaxis and augmented ROS levels in sera and bone marrow-derived mast cells (BMMCs). Furthermore, antigen-induced degranulation and production of TNF- and IL-4 were significantly amplified in DJ-1 KO and anti-DJ-1 small interfering RNA-transfected BMMCs compared with that seen in wild-type (WT) BMMCs. Studies with these cells and BMMCs transfected with small interfering RNAs against the phosphatases Src homology domain 2-containing protein tyrosine phosphatase (SHP) 1 and SHP-2 revealed that the DJ-1 KO phenotype could be attributed to suppression of SHP-1 activity and enhancement of SHP-2 activity, leading to strengthened signaling through linker for activation of T cells, phospholipase CÎ³, and mitogen-activated protein kinases. CONCLUSIONS: A deficiency or constitutive activation of DJ-1 can have implications in mast cell-driven allergic diseases, such as asthma and anaphylaxis.
File Format	HTM / HTML
ISSN	00916749
e-ISSN	10976825
DOI	10.1016/j.jaci.2012.10.012
Journal	Journal of Allergy and Clinical Immunology
Issue Number	6
Volume Number	131
Language	English
Publisher	Elsevier
Publisher Date	2013-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Immunology Hypersensitivity, Immediate Immunology Metabolism Intracellular Signaling Peptides And Proteins Mast Cells Oncogene Proteins Adaptor Proteins, Signal Transducing Adolescent Animals Antigens Bone Marrow Cells Cell Degranulation Child, Preschool Cytokines Biosynthesis Dermatitis, Atopic Disease Models, Animal Genetics Interleukin-4 Blood Membrane Proteins Mice Mice, Knockout Passive Cutaneous Anaphylaxis Phosphoproteins Phosphorylation Protein Tyrosine Phosphatase, Non-receptor Type 11 Protein Tyrosine Phosphatase, Non-receptor Type 6 Protein-tyrosine Kinases Proto-oncogene Proteins C-fyn Rna Interference Reactive Oxygen Species Receptors, Ige Signal Transduction Tumor Necrosis Factor-alpha Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mutation of tyrosine 145 of lymphocyte cytosolic protein 2 protects mice from anaphylaxis and arthritis.

Mechanisms of mast cell signaling in anaphylaxis.

Gab2, via PI-3K, regulates ARF1 in FcÎµRI-mediated granule translocation and mast cell degranulation.

FcɛRI-mediated mast cell degranulation requires calcium-independent microtubule-dependent translocation of granules to the plasma membrane

Coupling of Gab1 to C-Met, Grb2, and Shp2 Mediates Biological Responses

AMP-activated protein kinase negatively regulates FcÎµRI-mediated mast cell signaling and anaphylaxis in mice.

Direct Regulation of Nephrin Tyrosine Phosphorylation by Nck Adaptor Proteins

The adaptor 3BP2 is required for early and late events in FcÎµRI signaling in human mast cells.

Modulation of FcÎµRI-dependent mast cell response by OX40L via Fyn, PI3K, and RhoA.

DJ-1 regulates mast cell activation and IgE-mediated allergic responses.

Similar Documents

Mutation of tyrosine 145 of lymphocyte cytosolic protein 2 protects mice from anaphylaxis and arthritis.

Mechanisms of mast cell signaling in anaphylaxis.

Gab2, via PI-3K, regulates ARF1 in FcÎµRI-mediated granule translocation and mast cell degranulation.

FcɛRI-mediated mast cell degranulation requires calcium-independent microtubule-dependent translocation of granules to the plasma membrane

Coupling of Gab1 to C-Met, Grb2, and Shp2 Mediates Biological Responses

AMP-activated protein kinase negatively regulates FcÎµRI-mediated mast cell signaling and anaphylaxis in mice.

Direct Regulation of Nephrin Tyrosine Phosphorylation by Nck Adaptor Proteins

The adaptor 3BP2 is required for early and late events in FcÎµRI signaling in human mast cells.

Modulation of FcÎµRI-dependent mast cell response by OX40L via Fyn, PI3K, and RhoA.

DJ-1 regulates mast cell activation and IgE-mediated allergic responses.