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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Patra, Animesh Hazra, Soumitra Samanta, Nirnay Suresh Kumar, Gopinatha Mitra, Rajib Kumar |
| Description | Country affiliation: India Author Affiliation: Patra A ( Department of Chemical Biological and Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India.); Hazra S ( Biophysical Chemistry Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700 032, India.); Samanta N ( Department of Chemical Biological and Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India.); Suresh Kumar G ( Biophysical Chemistry Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700 032, India. Electronic address: gskumar@iicb.res.in.); Mitra RK ( Department of Chemical Biological and Macromolecular Sciences, S.N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India. Electronic address: rajib@bose.res.in.) |
| Abstract | We investigate the SDS micelle induced dissociation of a small fluorescent ligand 4',6-diamidino-2-phenylindole (DAPI) bound to DNAs of varying sequences. Steady state and time resolved fluorescence measurements affirm minor groove binding of DAPI to poly(dA).poly(dT) and calf thymus DNA while it intercalates in poly(dG).poly(dC). Calorimetric measurements identify the former mode to be entropy driven and the intercalation to be enthalpy driven. Addition of SDS micelles extracts the ligand out of the DNA and relocates it into the micelle independent of the DNA-ligand binding mode. This process is found to be endothermic which is compensated by a huge gain in the entropy. Circular dichroism measurements indicate that the micelles do not affect the structure of DNAs, however, binding and un-binding of DAPI can introduce noticeable alteration in the DNA structure and consequently on the associated hydration which is reflected in solvation measurement. Consideration of a simple two step equilibrium model seems inadequate to account for the observed thermodynamic costs in the dissociation process. The results have been discussed on the basis of an intricate enthalpy-entropy balance. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 82 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Dna Chemistry Ligands Micelles Animals Calorimetry Cattle Circular Dichroism Nucleic Acid Conformation Thermodynamics Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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