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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Housley, Michael P. Reischauer, Sven Dieu, Marc Raes, Martine Stainier, Didier Y. R. Vanhollebeke, Benoit |
| Description | Author Affiliation: Housley MP ( Department of Biochemistry & Biophysics, UCSF, San Francisco, CA 94158, USA michael.housley@mpi-bn.mpg.de didier.stainier@mpi-bn.mpg.de benoit.vanhollebeke@ulb.ac.be.); Reischauer S ( Department of Biochemistry & Biophysics, UCSF, San Francisco, CA 94158, USA.); Dieu M ( URBC-Narilis, University of Namur, Namur B-5000, Belgium.); Raes M ( URBC-Narilis, University of Namur, Namur B-5000, Belgium.); Stainier DY ( Department of Biochemistry & Biophysics, UCSF, San Francisco, CA 94158, USA michael.housley@mpi-bn.mpg.de didier.stainier@mpi-bn.mpg.de benoit.vanhollebeke@ulb.ac.be.); Vanhollebeke B ( Department of Biochemistry & Biophysics, UCSF, San Francisco, CA 94158, USA michael.housley@mpi-bn.mpg.de didier.stainier@mpi-bn.mpg.de benoit.vanhollebeke@ulb.ac.be.) |
| Abstract | Heterogeneity within a population of cells of the same type is a common theme in metazoan biology. Dissecting complex developmental and physiological processes crucially relies on our ability to probe the expression profile of these cell subpopulations. Current strategies rely on cell enrichment based on sequential or simultaneous use of multiple intersecting markers starting from a heterogeneous cell suspension. The extensive tissue manipulations required to generate single-cell suspensions, as well as the complexity of the required equipment, inherently complicate these approaches. Here, we propose an alternative methodology based on a genetically encoded system in the model organism Danio rerio (zebrafish). In transgenic fish, we take advantage of the combinatorial biotin transfer system, where polysome-associated mRNAs are selectively recovered from cells expressing both a tagged ribosomal subunit, Rpl10a, and the bacterial biotin ligase BirA. We have applied this technique to skeletal muscle development and identified new genes with interesting temporal expression patterns. Through this work we have thus developed additional tools for highly specific gene expression profiling. |
| File Format | HTM / HTML |
| ISSN | 09501991 |
| e-ISSN | 14779129 |
| DOI | 10.1242/dev.111849 |
| Journal | Development |
| Issue Number | 20 |
| Volume Number | 141 |
| Language | English |
| Publisher | The Company of Biologists |
| Publisher Date | 2014-10-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Discipline Developmental Discipline Biology Gene Expression Profiling Gene Expression Regulation, Developmental Rna-binding Proteins Physiology Transcription, Genetic Zebrafish Proteins Animals Animals, Genetically Modified Biotinylation Coenzyme A Ligases Chemistry Green Fluorescent Proteins In Situ Hybridization Mass Spectrometry Muscle, Skeletal Pathology Polyribosomes Rna, Messenger Metabolism Ribosomal Proteins Ribosomes Zebrafish Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Developmental Biology Molecular Biology |
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