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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pinto, Mariana Coelho, Elisabete Nunes, Alexandra Brandão, Tiago Coimbra, Manuel A. |
| Description | Country affiliation: Portugal Author Affiliation: Pinto M ( QOPNA, Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal.); Coelho E ( QOPNA, Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal. Electronic address: ecoelho@ua.pt.); Nunes A ( Centro de Biologia Celular, Departamento de Biologia, Secção Autónoma de Ciências da Saúde, Universidade de Aveiro, 3810-193 Aveiro, Portugal.); Brandão T ( Unicer Bebidas, SA, Leça do Balio, 4466-955 S. Mamede de Infesta, Portugal.); Coimbra MA ( QOPNA, Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal.) |
| Abstract | Brewers spent yeast (BSY) is a by-product from beer industry that can be exploited as source of glucans and mannoproteins, with potential biological activities. In order to solubilize these carbohydrate-rich polymeric materials, a sequential extraction with hot water and alkaline solutions (0.1-8 M KOH) was performed. Mannoproteins were mainly (85%) extracted with 4 M KOH whereas glucans were extracted with 8 M KOH and in an amount that accounted only for 34% of total glucose. Final residue still accounted for 34% of the initial glucans and contained 98% of glucose. Cellulase and -amylase treatments showed the presence of both - and ß-(1â4)-Glc linkages. To promote total solubilization of these insoluble glucans, the final residue was submitted to a partial acid hydrolysis. This work is the first report showing that the most abundant polysaccharides in BSY are polymers that contain structural features similar to cellulose, thus justifying their resistance to alkaline extractions, acid hydrolysis, and insolubility in water. |
| File Format | HTM / HTML |
| ISSN | 01448617 |
| Volume Number | 116 |
| e-ISSN | 18791344 |
| Journal | Carbohydrate Polymers |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-02-13 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry__semicolon__materials Discipline Science Polysaccharides Chemistry Saccharomyces Cerevisiae Beer Chemical Fractionation Chromatography, Gel Food Industry Glycoside Hydrolases Hydrolysis Hydroxides Industrial Waste Potassium Compounds Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Materials Chemistry Polymers and Plastics |
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