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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, Xiaolei Li, Dan |
| Description | Author Affiliation: Li X ( Key Laboratory of Agroproducts Processing Technology at Jilin Provincial Universities, Education Department of Jilin Provincial Government, and College of Food Science and Engineering, Changchun University, Satellite Road 6543, Changchun 130022, Jilin, People's Republic of China.); Li D ( Key Laboratory of Agroproducts Processing Technology at Jilin Provincial Universities, Education Department of Jilin Provincial Government, and College of Food Science and Engineering, Changchun University, Satellite Road 6543, Changchun 130022, Jilin, People's Republic of China) |
| Abstract | A novel method for the preparation of linear maltodextrins from cyclodextrins and starch was proposed. To accomplish this process, an amylopullulanase from hyperthermophilic archaeon Caldivirga maquilingensis (CMApu) was characterized and used. CMApu with an estimated molecular mass of 62.7 kDa by SDS-PAGE had a maximal pullulan-hydrolysing activity at 100°C and pH 5.0. It could also hydrolyse amylopectin (AP), starch, ß-CD and amylose (AM), in a decreasing order of relative activities from 88.96% to 57.17%. TLC and HPAEC analysis revealed that CMApu catalyzed the debranching and degrading reactions to produce linear malto-oligosaccharides (≤ G8-G1) from G8-ß-CD and/or normal CDs, amylodextrins (DP6-96) from AM, and amylodextrins (DP1-76) from AP and potato starch. Our results showed that CMApu had a great potential for the industrial preparation of linear maltodextrins from normal starch instead of waxy starch, malto-oligosaccharides or sucrose. And the high optimal temperature of CMApu facilitated the simultaneous gelatinization and hydrolysis of cereal starch. |
| File Format | HTM / HTML |
| ISSN | 01448617 |
| Volume Number | 119 |
| e-ISSN | 18791344 |
| Journal | Carbohydrate Polymers |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-03-30 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry__semicolon__materials Discipline Science Cyclodextrins Metabolism Glycoside Hydrolases Polysaccharides Starch Temperature Archaea Enzymology Chromatography, Ion Exchange Chromatography, Thin Layer Conserved Sequence Electrophoresis, Polyacrylamide Gel Isolation & Purification Hydrogen-ion Concentration Hydrolysis Oligosaccharides Sequence Homology, Amino Acid Substrate Specificity Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Materials Chemistry Polymers and Plastics |
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