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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Albani, Silvia Maria Ferreira da Silva, Mateus Ribeiro Fratelli, Fernando Junior, Celso Preto Cardoso Iourtov, Dmitri Cintra, Felipe de Oliveira Takagi, Mickie Cabrera-Crespo, Joaquin |
| Description | Country affiliation: Brazil Author Affiliation: Albani SM ( Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Brazil.); da Silva MR ( Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Brazil.); Fratelli F ( Laboratório Especial Piloto de Produtos Biológicos Recombinantes, Instituto Butantan, Brazil.); Junior CP ( Laboratório Especial Piloto de Produtos Biológicos Recombinantes, Instituto Butantan, Brazil.); Iourtov D ( Divisão de Desenvolvimento Tecnológico e Produção, Instituto Butantan, Brazil.); Cintra Fde O ( Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Brazil.); Takagi M ( Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Brazil. Electronic address: mickie.takagi@butantan.gov.br.); Cabrera-Crespo J ( Laboratório de Bioprocessos, Centro de Biotecnologia, Instituto Butantan, Brazil.) |
| Abstract | Haemophilus influenzae type b (Hib) is a human pathogen that causes meningitis in infants worldwide. Capsular polysaccharide linked to a protein has been used as an efficient vaccine, and this approach has reduced the incidence of Hib disease since its inclusion in national immunisation campaigns. The traditional polysaccharide downstream process is based on several ethanol precipitations, treatment with detergents and centrifugation. The aim of this study was to introduce tangential microfiltration (TMF) in the place of centrifugation to simplify handling and to scale up the process. The purity of the polysaccharide was RPNA=1747.2 and RPPrt=196.1 for nucleic acid and protein, respectively, meeting the quality requirements for this polysaccharide. Moreover, the polysaccharide was recognised by at specific antibody, and the ribose and phosphate contents were within the expected limits. Thus, we established a process for the purification of capsular polysaccharide produced by H. influenzae type b that is effective, robust and feasible to be scaling up. |
| File Format | HTM / HTML |
| ISSN | 01448617 |
| Volume Number | 116 |
| e-ISSN | 18791344 |
| Journal | Carbohydrate Polymers |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-02-13 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry__semicolon__materials Discipline Science Haemophilus Influenzae Type b Polysaccharides, Bacterial Isolation & Purification Bacterial Proteins Analysis Bioreactors Chemical Precipitation Filtration Metabolism Nucleic Acids Phosphorus Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Materials Chemistry Polymers and Plastics |
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