| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Ropartz, David Lemoine, Jérôme Giuliani, Alexandre Bittebière, Yann Enjalbert, Quentin Antoine, Rodolphe Dugourd, Philippe Ralet, Marie-Christine Rogniaux, Hélène |
| Description |
Author Affiliation: Ropartz D ( INRA, UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France. Electronic address: David.Ropartz@nantes.inra.fr.); Lemoine J ( Institut des Sciences Analytiques, UMR 5280, Université Lyon 1-CNRS, Université de Lyon, F-69622 Villeurbanne cedex, France.); Giuliani A ( Synchrotron SOLEIL, L'Orme des Merisiers, F-91190 Gif-sur-Yvette, France); Bittebière Y ( INRA, UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France.); Enjalbert Q ( Institut des Sciences Analytiques, UMR 5280, Université Lyon 1-CNRS, Université de Lyon, F-69622 Villeurbanne cedex, France); Antoine R ( Institut Lumière Matière, UMR5306, Université Lyon 1-CNRS, Université de Lyon, F-69622 Villeurbanne cedex, France.); Dugourd P ( Institut Lumière Matière, UMR5306, Université Lyon 1-CNRS, Université de Lyon, F-69622 Villeurbanne cedex, France.); Ralet MC ( INRA, UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France.); Rogniaux H ( INRA, UR1268 Biopolymers Interactions Assemblies, F-44316 Nantes, France.) |
| Abstract |
Carbohydrates have a wide variety of structures whose complexity and heterogeneity challenge the field of analytical chemistry. Tandem mass spectrometry, with its remarkable sensitivity and high information content, provides key advantages to addressing the structural elucidation of polysaccharides. Yet, classical fragmentation by collision-activated dissociation (CAD) in many cases fails to reach a comprehensive structural determination, especially when isomers have to be differentiated. In this work, for the first time, vacuum ultra-violet (VUV) synchrotron radiation is used as the activation process in tandem mass spectrometry of large oligosaccharides. Compared to low energy CAD (LE-CAD), photon activated dissociation brought more straightforward and valuable structural information. The outstanding feature was that complete series of informative ions were produced, with only minor neutral losses. Moreover, systematic fragmentation rules could be drawn thus facilitating the definitive assignments of fragment identities. As a result, most of the structures present in a complex mixture of oligogalacturonans could be comprehensively resolved, including many isomers differing in the position of methyl groups along the galacturonic acid backbone. |
| File Format |
HTM / HTML |
| ISSN |
00032670 |
| Volume Number |
807 |
| e-ISSN |
18734324 |
| Journal |
Analytica Chimica Acta |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-01-07 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Analytical Discipline Chemistry Oligosaccharides Chemistry Tandem Mass Spectrometry Chromatography, High Pressure Liquid Chromatography, Reverse-phase Ions Isomerism Photons Spectrometry, Mass, Electrospray Ionization Synchrotrons Ultraviolet Rays Vacuum Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
