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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rühmann, Broder Schmid, Jochen Sieber, Volker |
| Description | Country affiliation: Germany Author Affiliation: Rühmann B ( Chemistry of Biogenic Resources, Technische Universität München, Germany.); Schmid J ( Chemistry of Biogenic Resources, Technische Universität München, Germany.); Sieber V ( Chemistry of Biogenic Resources, Technische Universität München, Germany. Electronic address: Sieber@tum.de.) |
| Abstract | A fast carbohydrate screening platform processible in 96-well format is described. The method is suitable for the determination of various carbohydrates out of complex mixtures as obtained by acidic hydrolysis of carbohydrates polymers. The chromatographic conditions for an efficient separation (12min) and the derivatization process with 1-phenyl-3-methyl-5-pyrazolone (PMP) were optimized for high resolution separation and simultaneous determination of deoxy-, amino-, anhydro-sugars as well as hexoses, pentoses, dimers, uronic acids and degradation products like furfural and hydroxymethylfurfural (HMF). The potential to quantify with UV- and MS-detector in the same range has been demonstrated for 20 different compounds. Finally, the matrix effects of the hydrolysis were positively evaluated. The micro scale hydrolysis and PMP-derivatization without any extraction or drying steps, both in 96-well format, result in a fast and intuitive sample preparation. In combination with a fast liquid chromatography coupled to UV and electrospray ionization ion trap detection (LC-UV-ESI-MS/MS) for the qualification and quantification of various sugars, dimers and degradation products, this method shows great performance in carbohydrate analysis. |
| ISSN | 00219673 |
| Volume Number | 1350 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-07-11 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Carbohydrates Analysis Chemistry Techniques, Analytical Methods Chromatography, Liquid Spectrometry, Mass, Electrospray Ionization Spectrophotometry, Ultraviolet Standards Reproducibility Of Results Journal Article Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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