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| Content Provider | Tech Science Press |
|---|---|
| Author | Cuitiño, M.j. Ortiz, A. M. Pizarro, M. A. Renna, N. F. Rüttler, M.e. Yanzón, C.s. |
| Abstract | Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator ( aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 ( astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a multiplex PCR assay which checks aggR and astA genes was designed. Eigthy- eight E.coli strains, isolated from children with acute diarrhea in Mendoza, Argentina, were characterized by the reference method (HEp-2 assay), and by aggRastA PCR. A strong correlation between the presence of the specific marker aggR and the reference test was found. The astA gene had a similar distribution between aggregative and localized strains, indicating that this gene could not be considered as a marker of EAEC. We conclude that aggR may be used to identify EAEC, using the PCR method as a screening test. |
| Related Links | https://www.techscience.com/biocell/v30n2/37695 |
| Starting Page | 301 |
| Ending Page | 308 |
| ISSN | 03279545 |
| DOI | 10.32604/biocell.2006.30.301 |
| Issue Number | 2 |
| Journal | BIOCELL (BIOCELL) |
| Volume Number | 30 |
| e-ISSN | 16675746 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Escherichia coli diarrhea PCR (Polymerase Chain Reaction) cell culture |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology |
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