NDLI: Detection of Escherichia Coli in Children Stool with Diarrhea Patients Using Culture and Polymerase Chain Reaction Method

Please wait, while we are loading the content...

Detection of Escherichia Coli in Children Stool with Diarrhea Patients Using Culture and Polymerase Chain Reaction Method

Content Provider	Semantic Scholar
Author	Purnamasari, Lokita Massi, Muh Nasrum Ahmad, Ahyar
Copyright Year	2017
Abstract	ABSTRACT Introduction : Diarrheal diseases are a serious issue on health in developing countries and the liquid cause's morbidity and mortality in children that cause the diarrhea pathogenic bacte- ria including Escherichia coli. This research aims to detect E. coli in stool diarrhea patient of the child with culture method and Polymerase Chain Reaction. Methods : This research is descriptive research design with cross-sectional approach. The sampling was collected from some public health centers namely Pampang, Barabarayya, Antang Perumnas, Tamangapa public health centers. Specimen testing process was conducted at Microbiology laboratory of the Teaching Hospital University of Hasanuddin to identify E. coli bacterium with the culture and to detect eae gene and bfp with PCR technique. Results : The results indicate that from 50 samples, there are 15 samples (30%) of samples positively detection E. coli using culture method and PCR test using eae and bfp primer found 20 (40%) and 1 sample (2%) positive enteropathogen E. coli. Conclusion : PCR methods indicated the result of E. coli bacteria results faster and more accurate that other culture methods. Keywords : Diarrhea, Escherichia coli, Polymerase Chain Reaction
Starting Page	22
Ending Page	27
Page Count	6
File Format	PDF HTM / HTML
DOI	10.20956/nmsj.v2i1.2256
Volume Number	2
Alternate Webpage(s)	http://journal.unhas.ac.id/index.php/jmednus/article/download/2256/1248
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in