NDLI: A pseudo-outbreak of MRSA due to laboratory contamination related to MRSA carriage of a laboratory staff member

Content Provider	Springer Nature : BioMed Central
Author	Houkes, Karlijn M. G. Stohr, Joep J. J. M. Gast, Karin B. Couderé, Karen Weterings, Veronica Mutsaers - van Oudheusden, Anne Buiting, Anton G. M. Verweij, Jaco J.
Abstract	Background Methicillin resistant Staphylococcus aureus (MRSA) is a major burden for hospitals globally. However, in the Netherlands, the MRSA prevalence is relatively low due to the ‘search and destroy’ policy. Routine multiple-locus variable-number of tandem repeat analysis (MLVA) of MRSA isolates supports outbreak detection. However, whole genome multiple locus sequence typing (wgMLST) is superior to MLVA in identifying (pseudo-)outbreaks with MRSA. The present study describes a pseudo-outbreak of MRSA at the bacteriology laboratory of a large Dutch teaching hospital. Methods All staff members of the bacteriology laboratory of the Elisabeth-TweeSteden hospital were screened for MRSA carriage, after a laboratory contamination with MRSA was suspected. Clonal relatedness between the index isolate and the MRSA isolates from laboratory staff members and all previous MRSA isolates from the Elisabeth-TweeSteden hospital with the same MLVA-type as the index case was examined based on wgMLST using whole genome sequencing. Results One of the staff members was identified as the probable source of the laboratory contamination, because of carriage of a MRSA possessing the same MLVA-type as the index case. Eleven other isolates with the same molecular characteristics were found in the database, of which seven were retrospectively suspected of contamination. Clonal relatedness was found between ten isolates, including the isolate found in the staff member and the MRSA found in the index patient with a maximum of eleven alleles difference. All isolates were epidemiologically linked through the laboratory staff member, who had worked on all these cultures. Conclusions The present study describes a MRSA pseudo-outbreak over a 2.5-year period due to laboratory contamination caused by a MRSA carrying laboratory staff member involving nine patients. In case of unexpected bacteriological findings, the possibility of a laboratory contamination should be considered.
Related Links	https://aricjournal.biomedcentral.com/counter/pdf/10.1186/s13756-022-01207-7.pdf
Ending Page	7
Page Count	7
Starting Page	1
File Format	HTM / HTML
ISSN	20472994
DOI	10.1186/s13756-022-01207-7
Journal	Antimicrobial Resistance & Infection Control
Issue Number	1
Volume Number	12
Language	English
Publisher	BioMed Central
Publisher Date	2023-01-05
Access Restriction	Open
Subject Keyword	Medical Microbiology Drug Resistance Infectious Diseases MRSA Pseudo-outbreak Contamination wgMLST
Content Type	Text
Resource Type	Article
Subject	Public Health, Environmental and Occupational Health Infectious Diseases Microbiology (medical) Pharmacology (medical)
Journal Impact Factor	4.8/2023
5-Year Journal Impact Factor	5.8/2023

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