NDLI: Differential airway resistome and its correlations with clinical characteristics in Haemophilus- or Pseudomonas-predominant microbial subtypes of bronchiectasis

Content Provider	Springer Nature : BioMed Central
Author	Yi, Xin-zhu Yang, Jun-hao Huang, Yan Han, Xiao-rong Li, Hui-min Cen, Lai-jian Lin, Zhen-hong Pan, Cui-xia Wang, Zhang Guan, Wei-jie
Abstract	The prevalence and clinical correlates of antibiotic resistance genes (ARGs) in bronchiectasis are not entirely clear. We aimed to profile the ARGs in sputum from adults with bronchiectasis, and explore the association with airway microbiome and disease severity and subtypes. In this longitudinal study, we prospectively collected 118 sputum samples from stable and exacerbation visits of 82 bronchiectasis patients and 19 healthy subjects. We profiled ARGs with shotgun metagenomic sequencing, and linked these to sputum microbiome and clinical characteristics, followed by validation in an international cohort. We compared ARG profiles in bronchiectasis according to disease severity, blood and sputum inflammatory subtypes. Unsupervised clustering revealed a Pseudomonas predominant subgroup (n = 16), Haemophilus predominant subgroup (n = 48), and balanced microbiome subgroup (N = 54). ARGs of multi-drug resistance were over-dominant in the Pseudomonas-predominant subgroup, while ARGs of beta-lactam resistance were most abundant in the Haemophilus-predominant subgroup. Pseudomonas-predominant subgroup yielded the highest ARG diversity and total abundance, while Haemophilus–predominant subgroup and balanced microbiota subgroup were lowest in ARG diversity and total abundance. PBP-1A, ksgA and emrB (multidrug) were most significantly enriched in Haemophilus-predominant subtype. ARGs generally correlated positively with Bronchiectasis Severity Index, fluoroquinolone use, and modified Reiff score. 68.6% of the ARG-clinical correlations could be validated in an independent international cohort. In conclusion, ARGs are differentially associated with the dominant microbiome and clinical characteristics in bronchiectasis.
Related Links	https://respiratory-research.biomedcentral.com/counter/pdf/10.1186/s12931-023-02562-8.pdf
Ending Page	12
Page Count	12
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/s12931-023-02562-8
Journal	Respiratory Research
Issue Number	1
Volume Number	24
Language	English
Publisher	BioMed Central
Publisher Date	2023-11-02
Access Restriction	Open
Subject Keyword	Pneumology Respiratory System Antibiotics Resistome Macrolides Disease severity Inflammation Bronchiectasis Pneumology/Respiratory System
Content Type	Text
Resource Type	Article
Subject	Pulmonary and Respiratory Medicine
Journal Impact Factor	4.7/2023
5-Year Journal Impact Factor	5.3/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Arterial stiffness in adults with steady-state bronchiectasis: association with clinical indices and disease severity

Microbiological characteristics of the lower airway in adults with bronchiectasis: a prospective cohort study

Understanding the effects of Haemophilus influenzae colonization on bronchiectasis: a retrospective cohort study

Risk factors for Pseudomonas aeruginosa colonization in non-cystic fibrosis bronchiectasis and clinical implications

Characteristics of different asthma phenotypes associated with cough: a prospective, multicenter survey in China

Airway inflammation and mannitol challenge test in COPD

Clinical impact of cardiovascular disease on patients with bronchiectasis

Comparative microbiome analysis in cystic fibrosis and non-cystic fibrosis bronchiectasis.

Nationwide survey of refractory asthma with bronchiectasis by inflammatory subtypes

Differential airway resistome and its correlations with clinical characteristics in Haemophilus- or Pseudomonas-predominant microbial subtypes of bronchiectasis

Similar Documents

Arterial stiffness in adults with steady-state bronchiectasis: association with clinical indices and disease severity

Microbiological characteristics of the lower airway in adults with bronchiectasis: a prospective cohort study

Understanding the effects of Haemophilus influenzae colonization on bronchiectasis: a retrospective cohort study

Risk factors for Pseudomonas aeruginosa colonization in non-cystic fibrosis bronchiectasis and clinical implications

Characteristics of different asthma phenotypes associated with cough: a prospective, multicenter survey in China

Airway inflammation and mannitol challenge test in COPD

Clinical impact of cardiovascular disease on patients with bronchiectasis

Comparative microbiome analysis in cystic fibrosis and non-cystic fibrosis bronchiectasis.

Nationwide survey of refractory asthma with bronchiectasis by inflammatory subtypes

Differential airway resistome and its correlations with clinical characteristics in Haemophilus- or Pseudomonas-predominant microbial subtypes of bronchiectasis