NDLI: Virulence of H5N1 virus in mice attenuates after in vitro serial passages

Content Provider	Springer Nature : BioMed Central
Author	Li, Jing Liu, Bohua Chang, Guohui Hu, Yi Zhan, Dawei Xia, Yukun Li, Yongqiang Yang, Yinhui Zhu, Qingyu
Abstract	The virulence of A/Vietnam/1194/2004 (VN1194) in mice attenuated after serial passages in MDCK cells and chicken embryos, because the enriched large-plaque variants of the virus had significantly reduced virulence. In contrast, the small-plaque variants of the virus and the variants isolated from the brain of mice that were infected with the parental virus VN1194 had much higher virulence in mice. The virulence attenuation of serially propagated virus may be caused by the reduced neurotropism in mice. Our whole genome sequence analysis revealed substitutions of a total of two amino acids in PB1, three in PB2, two in PA common for virulence attenuated variants, all or part of which may be correlated with the virulence attenuation and reduced neurotropism of the serially propagated VN1194 in mice. Our study indicates that serial passages of VN1194 in vitro lead to adaptation and selection of variants that have markedly decreased virulence and neurotropism, which emphasizes the importance of direct analysis of original or less propagated virus samples.
Related Links	https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-8-93.pdf
Ending Page	8
Page Count	8
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/1743-422X-8-93
Journal	Virology Journal
Issue Number	1
Volume Number	8
Language	English
Publisher	BioMed Central
Publisher Date	2011-03-04
Access Restriction	Open
Subject Keyword	Virology A549 Cell H5N1 Virus MDCK Cell Avian Influenza Virus Serial Passage
Content Type	Text
Resource Type	Article
Subject	Virology Infectious Diseases
Journal Impact Factor	4/2023
5-Year Journal Impact Factor	3.8/2023

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