NDLI: Sequence analysis of fusion protein gene of Newcastle disease virus isolated from outbreaks in Egypt during 2006

Content Provider	Springer Nature : BioMed Central
Author	Mohamed, Mahmoud HA Kumar, Sachin Paldurai, Anandan Samal, Siba K
Abstract	Background Newcastle disease virus represents APMV-1 and is the most characterized among all APMV types. The F protein cleavage site sequence is a well-characterized determinant of NDV pathogenicity in chickens. In this study, the sequences of fusion protein (F) gene of three Newcastle disease virus (NDV) strains isolated from outbreak in chickens in the Al-Sharkia province of Egypt in 2006 were determined. Findings The viral genomic RNAs were extracted from the infective allantoic fluid and F gene is amplified using primer sets designed from the available sequences of NDV strains from GenBank. The pathogenicity of NDV strains was determined by three internationally recognized tests mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index. The phylogenetic analysis showed that the Egypt isolates are closely related with the genotype II of class II NDV strains. Conclusions The sequences of the F genes of the 2006 Egypt isolates are closely related to that of the 2005 Egypt isolate from the same province suggesting that these strains are probably circulating in the vaccinated bird population in Egypt until development of an outbreak.
Related Links	https://virologyj.biomedcentral.com/counter/pdf/10.1186/1743-422X-8-237.pdf
Ending Page	4
Page Count	4
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/1743-422X-8-237
Journal	Virology Journal
Issue Number	1
Volume Number	8
Language	English
Publisher	BioMed Central
Publisher Date	2011-05-18
Access Restriction	Open
Subject Keyword	Virology Newcastle disease virus African strain Chickens Sequence
Content Type	Text
Resource Type	Article
Subject	Virology Infectious Diseases
Journal Impact Factor	4/2023
5-Year Journal Impact Factor	3.8/2023

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