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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Tiuman, Tatiana Shioji Ueda-Nakamura, Tânia Alonso, Antonio Nakamura, Celso Vataru |
| Abstract | Background Leishmania amazonensis infection results in diverse clinical manifestations: cutaneous, mucocutaneous or visceral leishmaniasis. The arsenal of drugs available for treating Leishmania infections is limited. Therefore, new, effective, and less toxic leishmaniasis treatments are still needed. We verified cell death in amastigote forms of Leishmania amazonensis induced by the sesquiterpene lactone parthenolide. Results The tested compound was able to concentration-dependently affect axenic and intracellular amastigotes, with IC50 values of 1.3 μM and 2.9 μM, respectively after 72 h incubation. No genotoxic effects were observed in a micronucleus test in mice. Parthenolide induced morphological and ultrastructural changes in axenic amastigotes, including a loss of membrane integrity, swelling of the mitochondrion, cytoplasmic vacuoles, and intense exocytic activity in the region of the flagellar pocket. These results led us to investigate the occurrence of autophagic vacuoles with monodansylcadaverine and the integrity of the plasma membrane and mitochondrial membrane potential using flow cytometry. In all of the tests, parthenolide had positive results. Conclusions Our results indicate that the antileishmanial action of parthenolide is associated with autophagic vacuole appearance, a reduction of fluidity, a loss of membrane integrity, and mitochondrial dysfunction. Considering the limited repertoire of existing antileishmanial compounds, the products derived from medicinal plants has been one the greatest advances to help develop new chemotherapeutic approaches. |
| Related Links | https://bmcmicrobiol.biomedcentral.com/counter/pdf/10.1186/1471-2180-14-152.pdf |
| Ending Page | 12 |
| Page Count | 12 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 14712180 |
| DOI | 10.1186/1471-2180-14-152 |
| Journal | BMC Microbiology |
| Issue Number | 1 |
| Volume Number | 14 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2014-06-10 |
| Access Restriction | Open |
| Subject Keyword | Microbiology Biological Microscopy Mycology Parasitology Virology Life Sciences Leishmania amazonensis Amastigotes Parthenolide Cell death Autophagy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Microbiology (medical) |
| Journal Impact Factor | 4/2023 |
| 5-Year Journal Impact Factor | 4.6/2023 |
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