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  1. Journal of the Korean Society for Applied Biological Chemistry
  2. Journal of the Korean Society for Applied Biological Chemistry : Volume 57
  3. Journal of the Korean Society for Applied Biological Chemistry : Volume 57, Issue 1, February 2014
  4. Purification of an iron-chelating peptide from spirulina protein hydrolysates
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Journal of the Korean Society for Applied Biological Chemistry : Volume 60
Journal of the Korean Society for Applied Biological Chemistry : Volume 59
Journal of the Korean Society for Applied Biological Chemistry : Volume 58
Journal of the Korean Society for Applied Biological Chemistry : Volume 57
Journal of the Korean Society for Applied Biological Chemistry : Volume 57, Issue 6, December 2014
Journal of the Korean Society for Applied Biological Chemistry : Volume 57, Issue 5, October 2014
Journal of the Korean Society for Applied Biological Chemistry : Volume 57, Issue 4, August 2014
Journal of the Korean Society for Applied Biological Chemistry : Volume 57, Issue 3, June 2014
Journal of the Korean Society for Applied Biological Chemistry : Volume 57, Issue 2, April 2014
Journal of the Korean Society for Applied Biological Chemistry : Volume 57, Issue 1, February 2014
Degradation of limonene by gamma radiation for improving bioethanol production
Isolation and characterization of a biosurfactant-producing bacterium Bacillus pumilus IJ-1 from contaminated crude oil collected in Taean, Korea
Effect of soil water potential on methane and nitrous oxide emissions in upland soil during red pepper cultivation
Overexpression, purification, crystallization, and preliminary X-ray characterization of a methionine sulfoxide reductase AB from Helicobacter pylori
Standardized Rhus verniciflua stokes extract and its major flavonoid fustin attenuate oxidative stress induced by tert-butyl hydroperoxide via activation of nuclear factor erythroid 2-related factor
Potential of 4′-(p-toluenesulfonylamide)-4-hydroxychalcone to inhibit the human cytochrome P450 2J2 isoform
Activation of C2H2-type zinc finger genes induces dwarfism in Arabidopsis thaliana
Antioxidant capacities and cytostatic effect of Korean red pepper (Capsicum annuum L): a screening and in vitro study
Kinetics and modeling of pepsin soluble collagen (PSC) extraction from the skin of malaysian catfish (Hybrid Clarias sp.)
High-throughput HDR inhibitor screening
Levels of biogenic amines in cheeses and their impact on biochemical and histological parameters in rats
Establishment of analytical method for 6-benzylaminopurine residue, a plant growth regulator for brown rice, mandarin, pepper, potato, and soybean by using GC/NPD
Purification of an iron-chelating peptide from spirulina protein hydrolysates
Differential protein expression profiling in Pleurotus ferulae mycelium caused by asafoetida extracts using a proteomics approach
Antioxidant and antitumor activities of β-glucan-rich exopolysaccharides with different molecular weight from Paenibacillus polymyxa JB115
The inhibitory effects of the ethanolic extract of Pimpinella brachycarpa on cytochrome P450 enzymes in humans
Intracellular uranium accumulation by Shewanella sp. HN-41 under the thiosulfate-reducing condition
Microbially facilitated incorporation of As(III) into bioreduced Fe-(hydr)oxide minerals
A novel methoxyflavonol derivative, 2-hydroxy-3-(2-methoxyphenyl)-1H-benzo[f]chromen-1-one (DK98), induces apoptosis in HCT116 human colon cancer cells
Determination of capsaicin, ascorbic acid, total phenolic compounds and antioxidant activity of Capsicum annuum L. var. serrano by mid infrared spectroscopy (Mid-FTIR) and chemometric analysis
Journal of the Korean Society for Applied Biological Chemistry : Volume 56
Journal of the Korean Society for Applied Biological Chemistry : Volume 55
Journal of the Korean Society for Applied Biological Chemistry : Volume 54
Journal of the Korean Society for Applied Biological Chemistry : Volume 53
Journal of the Korean Society for Applied Biological Chemistry : Volume 52

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Purification of an iron-chelating peptide from spirulina protein hydrolysates

Content Provider Springer Nature Link
Author Kim, Nam Ho Kim, Jaehan Ahn, Hyun Joo Jung, Seung Hun Song, Kyung Bin Kim, Su Hee
Copyright Year 2014
Abstract Iron-chelating peptide was purified from spirulina protein hydrolysates. Spirulina protein was hydrolyzed using Alcalase and Flavourzyme, and the degree of hydrolysis was determined using a trinitrobenzene sulfonic acid assay and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The spirulina protein hydrolysates were ultra-filtered to isolate the components below 3 kDa, which were then fractionated by Q-Sepharose fast flow and Sephadex G-15 columns. The iron-chelating activity of each fraction was determined, and the peptide with the highest activity was isolated and identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. Amino acid sequence of the iron-chelating peptide was identified to be Thr-Asp-Pro-Ile(Leu)-Ala-Ala-Cys-Ile(Leu), which has a molecular weight of 802 Da. Moreover, due to its ability to chelate iron, the isolated peptide could be used as an iron supplement.
Starting Page 91
Ending Page 95
Page Count 5
File Format PDF
ISSN 17382203
Journal Journal of the Korean Society for Applied Biological Chemistry
Volume Number 57
Issue Number 1
e-ISSN 2234344X
Language English
Publisher The Korean Society for Applied Biological Chemistry
Publisher Date 2014-02-28
Publisher Place Seoul
Access Restriction Subscribed
Subject Keyword amino acid sequence spirulina purification Applied Microbiology Biological Techniques hydrolysates iron-chelating peptide Bioorganic Chemistry
Content Type Text
Resource Type Article
Subject Organic Chemistry Biochemistry, Genetics and Molecular Biology
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