NDLI: Characterization of artificially induced cadmium-tolerant yeast mutants

Content Provider	Springer Nature Link
Author	Lee, Sangman
Copyright Year	2014
Abstract	Bioremediation of heavy metals by using microorganisms is an effective strategy in regions that have low and wide-ranging metal concentrations and in situations where physical and chemical techniques are not suitable. Because of their higher capacity to remove a wide range of metals by biosorption, yeasts are useful for the bioremediation of heavy metals. In this study, identification of yeast mutants (CdRs) was focused, which have strong resistance to cadmium (Cd), a representative heavy metal. Yeast cells were sequentially adapted to gradually increasing the Cd concentration up to 30 mM. The resultant mutant, CdR30 cells survived a final Cd concentration of 30 mM, while the control cells failed to survive at 0.5 mM in 7 d. It was analyzed whether the increased Cd tolerance of the mutants was associated with sensitivity toward other metals. Compared to control cells, CdR20 cells showed increased tolerance to Cu, decreased tolerance to Ni, and comparable tolerance to Zn. However, these tolerances were not reproducible, because CdRs isolated in a second round of induction showed different metal sensitivities. The increase in Ni sensitivity in CdR20 cells was overcome by performing a second adaptation to Ni stress. Thus, CdR20 cells that were tolerant to both Cd and Ni were generated. These data presented in this study may be useful for the application of microorganisms to the bioremediation of heavy metals.
Starting Page	545
Ending Page	549
Page Count	5
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	57
Issue Number	5
e-ISSN	2234344X
Language	English
Publisher	The Korean Society for Applied Biological Chemistry
Publisher Date	2014-09-22
Publisher Place	Seoul
Access Restriction	Subscribed
Subject Keyword	bioremediation Applied Microbiology cadmium copper heavy metal Biological Techniques yeast Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

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