NDLI: Suppression of human hepatoma (HepG2) cell growth by nuclear factor-kappaB/p65 specific siRNA

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Tumor Biology : Volume 31, Issue 5, October 2010

Tumor Biology : Volume 31, Issue 4, August 2010

Tumor Biology : Volume 31, Issue 1, Supplement,August 2010

Tumor Biology : Volume 31, Issue 3, June 2010

Tumor Biology : Volume 31, Issue 2, April 2010

Tumor Biology : Volume 31, Issue 1, January 2010

Suppression of human hepatoma (HepG2) cell growth by nuclear factor-kappaB/p65 specific siRNA

Content Provider	Springer Nature Link
Author	Wu, Wei Yao, Dengfu Wang, Yilang Qiu, Liwei Sai, Wenli Yang, Junling Yao, Ninghua Li, Shanshan Bian, Yinzhu Wang, Zhiwei Yao, Dengbing
Copyright Year	2010
Abstract	Nuclear factor-kappaB (NF-κB) is a transcription factor and antagonist of apoptosis during liver regeneration and closely related to the formation and development of hepatocellular carcinoma. In the present study, we investigated the effect of small interference RNA (siRNA)-mediated inhibition of NF-κB on growth of human hepatoma (HepG2) cells. Our data indicated that the expression of NF-κB/p65 mRNA was significantly higher in the HepG2 cells than that in the normal liver (LO2) cells before transfection, and the expression of NF-κB/p65 in the HepG2 cells with NF-κB/p65 siRNA (100 nMol/L) transfection at 72 h was reduced at the levels of mRNA (93%) and protein (62%) using real-time reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and Western blotting. Interestingly, the apoptosis index of the HepG2 cells increased up to 85%, detected by Annexin V-fluorescein isothiocyanate, suggesting that NF-κB is overexpressed in hepatoma cells and can be inhibited by NF-κB/p65 siRNA through the apoptotic mechanism. Thus, we conclude that NF-κB is a potential molecular target for HCC gene therapy.
Starting Page	605
Ending Page	611
Page Count	7
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	31
Issue Number	6
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-07-14
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Hepatocellular carcinoma Nuclear factor-kappaB Apoptosis Gene therapy Real-time PCR Small interference RNA Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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