NDLI: Identification of OLIG2 as the most specific glioblastoma stem cell marker starting from comparative analysis of data from similar DNA chip microarray platforms

Content Provider	Springer Nature Link
Author	Trépant, Anne Laure Bouchart, Christelle Rorive, Sandrine Sauvage, Sébastien Decaestecker, Christine Demetter, Pieter Salmon, Isabelle
Copyright Year	2014
Abstract	Despite advances in surgical and adjuvant treatments, overall survival of glioblastoma (GBM) patients remains poor. The cancer stem cell concept suggests that a rare stem cell population, called glioma stem cells (GSCs), has high ability to self-renewal leading to recurrence in GBM. The identification of specific markers of GSCs would provide a powerful tool to detect and to characterise them in order to develop targeted therapies. We carried out a comparative analysis based on the identification of inter-study concordances to identify the genes that exhibit at best differential levels of expression between GSC-enriched cell cultures and differentiated tumour cell cultures from independent studies using DNA chip microarray technologies. We finally studied the protein expression of the marker we considered the most specific by immunohistochemistry and semi-quantitative analysis on a retrospective series of 18 GBMs. Of the selected studies, 32 genes were retained. Among them, eight genes were identified to be overexpressed in GSC-enriched cultures compared to differentiated tumour cell cultures. Finally, among the eight genes, oligodendrocyte lineage transcription factor 2 (OLIG2) was characterised by the most different expression level in the “GSC model” compared to the “differentiated tumour cells model”. Our approach suggests that OLIG2 is the most specific GSC marker; additional investigations with careful considerations about methodology and strategies of validation are, however, mandatory.
Starting Page	1943
Ending Page	1953
Page Count	11
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	36
Issue Number	3
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-11-12
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Olig2 Glioblastoma Glioma stem cells Microarray studies Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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