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| Content Provider | Springer Nature Link |
|---|---|
| Author | Gong, Peng Wang, Yingxin Wang, Yulin Jin, Shi Luo, Haifeng Zhang, Jing Bao, Haidong Wang, Zhongyu |
| Copyright Year | 2013 |
| Abstract | This study aims t explore the effect and application of bone marrow mesenchymal stem cells (BMSCs) on hepatocellular carcinoma in microcirculation by observing the angiogenesis of hepatocellular carcinoma in transplanted area. BMSCs were isolated and cultured primarily using the method of whole bone marrow culture and identifying surface antigens of third-generation bone marrow-derived mesenchymal stem cells using flow cytometry. Hepatoma cells cultured with BMSCs-conditioned medium (BMSCs-CM) were assayed using the cell proliferation rate of the MTT method. Nude mice were divided into control group (group A), BMSCs cell transplantation group (group B), HepG-2 cell group (group C), and combined BMSCs and HepG-2 cell cotransplanted group (group D). The result showed that the microvascular density was not significantly different in groups A and B. However, the microvascular density at 14 days was higher than 0 day in group C (P < 0.05). In group D, the microvascular density at 14 days was higher than that of 7 and 0 days (P < 0.05) and 7 days was higher than 0 days (P < 0.05). It was showed that the microvascular density did not get significant difference at 0 and 7 days in the four groups (P > 0.05). But the microvascular density of group C was higher than groups A and B at 14 days (P < 0.05), group D was higher than groups A and B at 14 days (P < 0.05) and group D was higher than group C at 14 days (P < 0.05). BMSCs could promote the growth of microvascular in hepatoma cells in a transplanted area. |
| Starting Page | 2161 |
| Ending Page | 2168 |
| Page Count | 8 |
| File Format | |
| ISSN | 10104283 |
| Journal | Tumor Biology |
| Volume Number | 34 |
| Issue Number | 4 |
| e-ISSN | 14230380 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2013-04-13 |
| Publisher Place | Dordrecht |
| Access Restriction | Subscribed |
| Subject Keyword | hepatocellular carcinoma BMSCs Dorsal skinfold chamber Microcirculation Cancer Research |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Cancer Research |
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