NDLI: Study of antagonism of citric acid on aluminum-induced toxicity in mice testis cells

Content Provider	Springer Nature Link
Author	Chen, Xiaoguang Deng, Wen Liu, Yumei Lv, Qiongxia
Copyright Year	2014
Abstract	To study the qualitative changes in testis tissue after aluminum chloride (AlCl$_{3}$) administration and to determine whether citric acid (CA) has a protective effect against testis damage induced by AlCl$_{3}$. In this study, 80 Kunming white mice were randomly separated into eight groups: (1) control, (2) CA (120 mg/kg), (3, 4 and 5) AlCl$_{3}$ (20, 40 and 60 mg/kg), (6, 7 and 8) AlCl$_{3}$ (20, 40 and 60 mg/kg) plus CA (120 mg/kg). After animals were killed, all testes were histopathologically examined under light microscopy; T-SOD and GSH-Px activities, H$_{2}$O$_{2}$ and MDA contents, and Bax and Bcl-2 levels were detected with the corresponding assay kits; DNA fragmentation were electrophoretically examined. Histopathological results indicated that AlCl$_{3}$ severely damage to mouse testis tissues, however, the protective effects on testes was observed when AlCl$_{3}$ combined with CA. Biochemical examination suggested that T-SOD and GSH-Px activities significantly decreased (P<0.05) in AlCl$_{3}$ groups, while remarkably improved in CA+AlCl$_{3}$ (especially middle and high dose groups) groups; H$_{2}$O$_{2}$ and MDA levels in higher-dose AlCl$_{3}$ groups were obviously higher (P<0.05) than in CA+ corresponding dose AlCl$_{3}$ groups. Cell apoptosis assays showed that Bax/Bcl-2 ratio in higher-dose AlCl$_{3}$ groups were higher than CA+ higher-dose AlCl$_{3}$ groups; and DNA fragmentation in middle-dose AlCl$_{3}$ groups was much more apparent in CA+ middle-dose AlCl$_{3}$ groups. It can be concluded that a certain concentration of AlCl$_{3}$ exerts a reproductive toxicity to mice, and administration of citric acid can reduce the adverse effects of AlCl$_{3}$ on testis.
Starting Page	443
Ending Page	450
Page Count	8
File Format	PDF
ISSN	1738642X
Journal	Molecular & Cellular Toxicology
Volume Number	10
Issue Number	4
e-ISSN	20928467
Language	English
Publisher	The Korean Society of Toxicogenomics and Toxicoproteomics
Publisher Date	2015-01-20
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Citric acid Aluminum chloride Testis Oxidative injury Cell apoptosis Cell Biology Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health Toxicology Pathology and Forensic Medicine

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