NDLI: Diversity and phylogenetic profiling of niche-specific Bacilli from extreme environments of India

Content Provider	Springer Nature Link
Author	Yadav, Ajar Nath Verma, Priyanka Kumar, Murugan Pal, Kamal K. Dey, Rinku Gupta, Alka Padaria, Jasdeep Chatrath Gujar, Govind T. Kumar, Sudheer Suman, Archna Prasanna, Radha Saxena, Anil K.
Copyright Year	2014
Abstract	The diversity of culturable, aerobic and heterotrophic Bacillus and Bacillus-derived genera (BBDG) was investigated in various extreme environments (including thermal springs, cold deserts, mangroves, salt lakes, arid regions, salt pans and acidic soils) of India. Heat treatment followed by enrichment in different media led to a total of 893 bacterial isolates. Amplified ribosomal DNA restriction analysis (ARDRA) using three restriction enzymes AluI, MspI and HaeIII led to the clustering of these isolates into 12–74 groups for the different sites at 75 % similarity index, adding up to 559 groups. Phylogenetic analysis based on 16S rRNA gene sequencing led to the identification of 392 bacilli, grouped in two families, Bacillaceae (89.03 %) and Paenibacillaceae (10.97 %), and included 13 different genera with 75 distinct species. It was found that among the thirteen genera, nine (Bacillus, Halobacillus, Lysinibacillus, Oceanobacillus, Pontibacillus, Salinibacillus, Sediminibacillus, Thalassobacillus and Virgibacillus) belonged to Bacillaceae and four (Ammoniphilus, Aneurinibacillus, Brevibacillus and Paenibacillus) belonged to Paenibacillaceae. Novel isolates tolerant to low and high pH and temperature, salt and low moisture were identified. The major outcome of the present investigation was the identification of niche-specific species and also the ubiquitous presence of selected species of BBDG, which illustrate the diversity and pervasive nature of BBDG in extreme environments.
Starting Page	611
Ending Page	629
Page Count	19
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	65
Issue Number	2
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-05-31
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	ARDRA Bacillus Bacillus derived genera 16S rRNA gene Extreme habitats Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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