NDLI: Rapid detection of neutral lipid in green microalgae by flow cytometry in combination with Nile red staining

Please wait, while we are loading the content...

Isolation and characterization of a protease-producing novel haloalkaliphilic bacterium Halobiforma sp. strain BNMIITR from Sambhar lake in Rajasthan, India

Evaluation of fermentative potential of Kluyveromyces marxianus ATCC 36907 in cellulosic and hemicellulosic sugarcane bagasse hydrolysates on xylitol and ethanol production

Antibiotic resistance and virulence of faecal enterococci isolated from food-producing animals in Tunisia

Composition and diversity of the bacterial community in snow leopard (Uncia uncia) distal gut

Overexpression of DosR in Mycobacterium tuberculosis does not affect aerobic replication in vitro or in murine macrophages

Gradual pediocin PA-1 resistance in Enterococcus faecalis confers cross-protection to diverse pore-forming cationic antimicrobial peptides displaying changes in cell wall and mannose PTS expression

Efficient production of ε-poly-l-lysine from agro-industrial by-products by Streptomyces sp. M-Z18

Decreased mineral availability enhances rock phosphate solubilization efficiency in Aspergillus niger

Screening of bioemulsifier-producing micro-organisms isolated from oil-contaminated sites

Morphological development of sclerotia by Sclerotinia sclerotiorum: a view from light and scanning electron microscopy

Improvement of the ability to produce spinosad in Saccharopolyspora spinosa through the acquisition of drug resistance and genome shuffling

Batch ethanol production from cassava (Manihot esculenta Crantz.) flour using Saccharomyces cerevisiae cells immobilized in calcium alginate

Molecular profiling of fungal assemblages in the healthy and infected roots of Decalepis arayalpathra (J. Joseph & V. Chandras) Venter, an endemic and endangered ethnomedicinal plant from Western Ghats, India

Molecular phylogeny and evogenomics of heterocystous cyanobacteria using rbcl gene sequence data

Thermophilic bacteria that tolerate a wide temperature and pH range colonize the Soldhar (95 °C) and Ringigad (80 °C) hot springs of Uttarakhand, India

Pterostilbene production by microorganisms expressing resveratrol O-methyltransferase

Mesophilic strains of Aeromonas spp. can acquire the multidrug resistance plasmid pRAS1 in horizontal transfer experiments at low temperatures

Hydrogen peroxide-mediated killing of Caenorhabditis elegans by Enterococcus italicus and Lactococcus garvieae isolated from food

Antimicrobial activity of Myrtus communis L. water-ethanol extract against meat spoilage strains of Brochothrix thermosphacta and Pseudomonas fragi in vitro and in meat

Epiphytic bacteria biodiversity in Brazilian Cerrado fruit and their cellulolytic activity potential

Overproduction and properties of lipase by a wild strain of Burkholderia lata LBBIO-BL02 using chicken fat

Contrasting spatiotemporal patterns and environmental drivers of diversity and community structure of ammonia oxidizers, denitrifiers, and anammox bacteria in sediments of estuarine tidal flats

Rapid metabolic profiling of developing Pseudomonas aeruginosa biofilms by high-resolution mass spectrometry fingerprinting

Leuconostoc mesenteroides SJRP55: a potential probiotic strain isolated from Brazilian water buffalo mozzarella cheese

Changes of diversity and population of yeasts during the fermentations by pure and mixed inoculation of Saccharomyces cerevisiae strains

Cider fermentation with three Williopsis saturnus yeast strains and volatile changes

Characterization, modification, and overexpression of 3-phosphoglycerate dehydrogenase in Corynebacterium glutamicum for enhancing l-serine production

Rapid detection of neutral lipid in green microalgae by flow cytometry in combination with Nile red staining—an improved technique

Characterization of plant growth-promoting bacteria associated with rice cropped in iron-stressed soils

Purification of β-xylosidase from Aspergillus tamarii using ground oats and a possible application on the fermented hydrolysate by Pichia stipitis

Metabolic capacities and toxigenic potential as key drivers of Bacillus cereus ubiquity and adaptation

Impact of a soil sampling strategy on the spatial distribution and diversity of arbuscular mycorrhizal communities at a small scale in two winter cover crop rotational systems

Comparison of bacterial diversity from solar salterns and a simulated laboratory study

Physiological comparison of cells with high and low alcohol dehydrogenase activities in bacterial populations consuming ethanol

Isolation and characterization of an endophytic bacterium, Bacillus megaterium BMN1, associated with root-nodules of Medicago sativa L. growing in Al-Ahsaa region, Saudi Arabia

Carbon demand, utilization, and metabolic diversity of bacterioplankton in the frontal regimes of the Indian sector of the Southern Ocean

Activity of crude and fractionated extracts by lactic acid bacteria (LAB) isolated from local dairy, meat, and fermented products against Staphylococcus aureus

Development of an antibiotic-free plasmid selection system based on thymine auxotrophy in Lactococcus lactis

Isolation, characterization and plant growth promotion effects of putative bacterial endophytes associated with sweet sorghum (Sorghum bicolor (L) Moench)

Nitrate removal by a novel autotrophic denitrifier (Microbacterium sp.) using Fe(II) as electron donor

Probiotic characteristics and in vitro compatibility of a combination of Bifidobacterium breve M-16 V, Bifidobacterium longum subsp. infantis M-63 and Bifidobacterium longum subsp. longum BB536

Adhesion of Lactobacillus reuteri strain Lr1 to equine epithelial cells and competitive exclusion of Clostridium difficile from the gastro-intestinal tract of horses

Biotransformation of acetophenone and its halogen derivatives by Yarrowia lipolytica strains

An insight into kinetics and thermodynamics of gamma-aminobutyric acid production by Enterococcus faecium CFR 3003 in batch fermentation

A preliminary stochastic model for managing microorganisms in a recirculating aquaculture system

Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation

Phenotypic and genotypic antibiotic resistance profiles of Escherichia coli O157 from cattle and slaughterhouse wastewater isolates

The minimal regulatory region necessary for the expression of the Penicillium griseoroseum plg1 gene

A simple molecular method for monitoring co-inoculated yeasts during wine fermentation

Rapid evaluation of three biocide treatments against the cyanobacterium Nostoc sp. PCC 9104 by color changes

Growth in the presence of sucrose may decrease attachment of some oral bacteria to abiotic surfaces

Fast detection of mycobacteriophages through conserved genes

Gene expression of proteolytic system of Lactobacillus helveticus H9 during milk fermentation

Styrene is sensed by the N-terminal PAS sensor domain of StyS, a double sensor kinase from the styrene-degrading bacterium Pseudomonas fluorescens ST

Manipulation of culture conditions for extensive extracellular catalase production by Exiguobacterium oxidotolerans T-2-2$^{T}$

Study of PROEXO product influence on infant microbiota in an in vitro colonic fermentation system

Variability and genetic structure of a natural population of Citrus psorosis virus

Annals of Microbiology : Volume 65, Issue 1, March 2015

Annals of Microbiology : Volume 64

Annals of Microbiology : Volume 63

Annals of Microbiology : Volume 62

Annals of Microbiology : Volume 61

Annals of Microbiology : Volume 60

Annals of Microbiology : Volume 59

Annals of Microbiology : Volume 58

Annals of Microbiology : Volume 57

Annals of Microbiology : Volume 56

Rapid detection of neutral lipid in green microalgae by flow cytometry in combination with Nile red staining—an improved technique

Content Provider	Springer Nature Link
Author	Satpati, Gour Gopal Pal, Ruma
Copyright Year	2014
Abstract	A staining protocol for rapid in situ detection of neutral lipid using flow cytometry in combination with Nile red staining was optimized. Staining efficiency was tested in terms of fluorescence intensity (% grandparent) in varied concentrations of Nile red and dimethyl sulfoxide (DMSO), with variable incubation period, temperature and pH level. The improved method was tested using two microalgae: Chlorella ellipsoidea and Chlorococcum infusionum. Maximum staining efficiency was recorded with a concentration of 5 μg mL$^{−1}$ Nile red and 40 % DMSO in a 15-min incubation at 40 °C for both taxa (pH 6.5). The forward (FSC) and side scatter (SSC) two-dimensional dot plot showed highly scattered cells containing neutral lipid. The coefficient of variation, standard deviation, mean and median values were determined for quantification of neutral lipid. We also applied this modified method to detect the elevated level of neutral lipid in nitrate (NaNO$_{3}$)-depleted cells; the efficiency of this technique was justified indicating a prominent 3- to 4-fold increase in neutral lipid in treated cells. Confocal images of stained cells also revealed accumulation of high levels of neutral lipid in treated microalgal cells.
Starting Page	937
Ending Page	949
Page Count	13
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	65
Issue Number	2
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-07-22
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Flow cytometry Nile red Nitrate depletion Novel staining method Physico-chemical parameters Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Rapid detection of neutral lipid in green microalgae by flow cytometry in combination with Nile red staining—an improved technique

An alternative high-throughput staining method for detection of neutral lipids in green microalgae for biodiesel applications

Automation of a Nile red staining assay enables high throughput quantification of microalgal lipid production

The application of flow cytometry in microbiological monitoring during winemaking: two case studies

Neutral red as a mediator for the enhancement of electricity production using a domestic wastewater double chamber microbial fuel cell

At high temperature lipid production in Ettlia oleoabundans occurs before nitrate depletion

Nile Red, an Alternative Fluorescence Method for Quantification of Neutral Lipids in Microalgae

Neutral Lipid Content and Biomass Production in Skeletonema marinoi (Bacillariophyceae) Culture in Response to Nitrate Limitation

Monitoring Rhodosporidium toruloides NCYC 921 batch fermentations growing under carbon and nitrogen limitation by flow cytometry

Rapid detection of neutral lipid in green microalgae by flow cytometry in combination with Nile red staining—an improved technique

Similar Documents

Rapid detection of neutral lipid in green microalgae by flow cytometry in combination with Nile red staining—an improved technique

An alternative high-throughput staining method for detection of neutral lipids in green microalgae for biodiesel applications

Automation of a Nile red staining assay enables high throughput quantification of microalgal lipid production

The application of flow cytometry in microbiological monitoring during winemaking: two case studies

Neutral red as a mediator for the enhancement of electricity production using a domestic wastewater double chamber microbial fuel cell

At high temperature lipid production in Ettlia oleoabundans occurs before nitrate depletion

Nile Red, an Alternative Fluorescence Method for Quantification of Neutral Lipids in Microalgae

Neutral Lipid Content and Biomass Production in Skeletonema marinoi (Bacillariophyceae) Culture in Response to Nitrate Limitation

Monitoring Rhodosporidium toruloides NCYC 921 batch fermentations growing under carbon and nitrogen limitation by flow cytometry

Rapid detection of neutral lipid in green microalgae by flow cytometry in combination with Nile red staining—an improved technique