NDLI: Overexpression of DosR in Mycobacterium tuberculosis does not affect aerobic replication in vitro or in murine macrophages

Content Provider	Springer Nature Link
Author	Flores Valdez, Mario Alberto Freches, Danielle Bruffaerts, Nicolas Romano, Marta Schoolnik, Gary Dolganov, Gregory Huygen, Kris
Copyright Year	2014
Abstract	Mycobacterium tuberculosis H37Rv, constitutively expressing a second copy of the transcriptional regulator DosR (Rv3133c) under control of the hsp60 promoter, was compared to wild-type M. tuberculosis for in vitro expression of the target genes of the DosR dormancy regulon, for its in vitro growth characteristics in liquid 7H9 culture medium, and for its capacity to replicate in murine macrophages. Under aerobic conditions, hsp60-driven DosR significantly induced the expression of 39 out of 44 DosR regulon genes, as assessed by real time qPCR. Increased DosR regulon gene transcription in vitro did not modify the capacity of the strain to grow under axenic conditions nor to infect murine macrophages as compared to unmodified wild-type bacteria.
Starting Page	713
Ending Page	720
Page Count	8
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	65
Issue Number	2
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-05-24
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Tuberculosis DosR Latency Macrophage Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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Overexpression of DosR in Mycobacterium tuberculosis does not affect aerobic replication in vitro or in murine macrophages