NDLI: Screening and molecular identification of lactic acid bacteria from gari and fufu and gari effluents

Content Provider	Springer Nature Link
Author	Ayodeji, Busayo D. Piccirillo, Clara Ferraro, Vincenza Moreira, Patrícia R. Obadina, Adewale O. Sanni, Lateef O. Pintado, Maria M. E.
Copyright Year	2016
Abstract	Bacterial strains were isolated from cassava-derived food products and, for the first time, from cassava by-products, with a focus on gari, a flour-like product, and the effluents from the production processes for gari and fufu (a dough also made from cassava flour). A total of 47 strains were isolated, all of which were tested to determine their resistance to acidic pH and to bile salt environments. Four of the 47 isolates tested positive in both environments, and these four isolates also showed antibacterial behaviour towards both Gram-positive and Gram-negative microbial pathogens (i.e. Methicillin-resistance Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Salmonella enteritidis, Escherichia coli, Escherichia coli (O157), Yersinia enterocolitica). In most cases, the antibacterial activity was related to bacteriocin production. Molecular identification analysis (16S rDNA and randomly amplified polymorphic DNA-PCR) revealed that the four isolates were different strains of the same species, Lactobacillus fermentum. These results demonstrate that bacteria isolated from cassava-derived food items and cassava by-products have interesting properties and could potentially be used as probiotics.
Starting Page	123
Ending Page	133
Page Count	11
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	67
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-12-12
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Cassava Lactic acid bacteria Lactobacillus fermentum Antibacterial Waste valorization Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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