NDLI: Optimization of fermentation conditions for enhancing extracellular production of L-asparaginase, an anti-leukemic agent, by newly isolated Streptomyces brollosae NEAE-115 using solid state fermentation

Please wait, while we are loading the content...

Optimization of fermentation conditions for enhancing extracellular production of L-asparaginase, an anti-leukemic agent, by newly isolated Streptomyces brollosae NEAE-115 using solid state fermentation

Hop resistance and beer-spoilage features of foodborne Bacillus cereus newly isolated from filtration-sterilized draft beer

Selection and evaluation of functional characteristics of autochthonous lactic acid bacteria isolated from traditional fermented stinky bean (Sataw-Dong)

Effects of irrigation treatments on the quality of table olives produced with the Greek-style process

A nodule endophytic plant growth-promoting Pseudomonas and its effects on growth, nodulation and metal uptake in Medicago lupulina under copper stress

Survey of mycobiota, black Aspergillus and ochratoxin A occurrence on Brazilian wine grapes

Purification, characterization and gene analysis of a new α-glucosidase from shiraia sp. SUPER-H168

Genetic analysis of arsenic metabolism in Micrococcus luteus BPB1, isolated from the Bengal basin

Bioreduction of hexavalent chromium by Pseudomonas stutzeri L1 and Acinetobacter baumannii L2

Characterisation of yeast microbiota, chemical and sensory properties of organic and biodynamic Sangiovese red wines

Development of a beverage from red grape juice fermented with the Kombucha consortium

Screening and molecular identification of lactic acid bacteria from gari and fufu and gari effluents

Diversity of species and antibiotic resistance in enterococci isolated from seafood in Tunisia

Annals of Microbiology : Volume 66

Annals of Microbiology : Volume 65

Annals of Microbiology : Volume 64

Annals of Microbiology : Volume 63

Annals of Microbiology : Volume 62

Annals of Microbiology : Volume 61

Annals of Microbiology : Volume 60

Annals of Microbiology : Volume 59

Annals of Microbiology : Volume 58

Annals of Microbiology : Volume 57

Annals of Microbiology : Volume 56

Optimization of fermentation conditions for enhancing extracellular production of L-asparaginase, an anti-leukemic agent, by newly isolated Streptomyces brollosae NEAE-115 using solid state fermentation

Content Provider	Springer Nature Link
Author	El Naggar, Noura Moawad, Hassan Abdelwahed, Nayera A. M.
Copyright Year	2016
Abstract	Optimization of the fermentation conditions for extracellular production of L-asparaginase by Streptomyces brollosae NEAE-115 under solid state fermentation was investigated. The Plackett–Burman experimental design was used to screen 16 independent variables (incubation time, moisture content, inoculum size, temperature, pH, soybean meal + wheat bran, dextrose, fructose, L-asparagine, yeast extract, KNO$_{3}$, K$_{2}$HPO$_{4}$, MgSO$_{4}$.7H$_{2}$O, NaCl, FeSO$_{4}$. 7H$_{2}$O, CaCl$_{2}$) and three dummy variables. The most significant independent variables found to affect enzyme production, namely soybean + wheat bran (X$_{6}$), L-asparagine (X$_{9}$) and K$_{2}$HPO$_{4}$ (X$_{12}$), were further optimized by the central composite design. We found that L-asparaginase production by S. brollosae NEAE-115 was 47.66, 129.92 and 145.57 units per gram dry substrate (U/gds) after an initial survey using “soybean meal + wheat bran” as a substrate for L-asparaginase production (step 1), statistical optimization by Plackett–Burman design (step 2) and further optimization by the central composite design (step 3), respectively, with a fold of increase of 3.05.
Starting Page	1
Ending Page	15
Page Count	15
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	67
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-08-09
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Streptomyces brollosae NEAE-115 L-asparaginase production Solid state fermentation Soybean meal Plackett–Burman design and central composite design Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115

Identification and statistical optimization of fermentation conditions for a newly isolated extracellular cholesterol oxidase-producing Streptomyces cavourensis strain NEAE-42

Process development for scale-up production of a therapeutic L-asparaginase by Streptomyces brollosae NEAE-115 from shake flasks to bioreactor.

L-Asparaginase Production using Solid-state Fermentation by an Endophytic Talaromyces pinophilus Isolated from Rhizomes of Curcuma amada

Optimization of fermentation conditions to increase the production of antifungal metabolites from Streptomyces sp. KN37

Optimization of Thermostable Amylopullulanase Production in Solid State Fermentation by Clostridium thermosulfurogenes SVM17 Through Plackett-Burman and Response Surface Methodological Approaches

Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology.

Streptomyces griseorubens as a microbial cell factory for extracellular uricase production and bioprocess optimization using statistical approach

Optimization of fermentation conditions for enhancing extracellular production of L-asparaginase, an anti-leukemic agent, by newly isolated Streptomyces brollosae NEAE-115 using solid state fermentation

Similar Documents

Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115

Identification and statistical optimization of fermentation conditions for a newly isolated extracellular cholesterol oxidase-producing Streptomyces cavourensis strain NEAE-42

Process development for scale-up production of a therapeutic L-asparaginase by Streptomyces brollosae NEAE-115 from shake flasks to bioreactor.

L-Asparaginase Production using Solid-state Fermentation by an Endophytic Talaromyces pinophilus Isolated from Rhizomes of Curcuma amada

Optimization of fermentation conditions to increase the production of antifungal metabolites from Streptomyces sp. KN37

Optimization of Thermostable Amylopullulanase Production in Solid State Fermentation by Clostridium thermosulfurogenes SVM17 Through Plackett-Burman and Response Surface Methodological Approaches

Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology.

Streptomyces griseorubens as a microbial cell factory for extracellular uricase production and bioprocess optimization using statistical approach

Optimization of fermentation conditions for enhancing extracellular production of L-asparaginase, an anti-leukemic agent, by newly isolated Streptomyces brollosae NEAE-115 using solid state fermentation