NDLI: Diversity of bacteria and polyketide synthase associated with marine sponge Haliclona sp.

Content Provider	Springer Nature Link
Author	Khan, Shams Tabrez Musarrat, Javed Alkhedhairy, Abdulaziz A. Kazuo, Shinya
Copyright Year	2013
Abstract	The microbial community associated with a marine sponge (Haliclona sp.) collected from Tateyama city, Japan was studied using 16S rRNA gene clone libraries. Two DNA templates were prepared using methods recommended for Gram-positive and Gram-negative bacteria in the Qiagen kit manual. From each DNA template, two 16S rRNA genes were PCR amplified, using the combination of universal bacterial primer 27f and primers 1385r and 1492r, respectively. A total of 347 clones were sequenced and compared with those available in DNA data banks. These sequences were members of ten bacterial phyla. Interestingly, more than 30 % of the clones represent novel sequences. A comparison of these sequences with sequences in a library prepared from DNA extracted from the surrounding water shows minimum DNA contamination. Taxonomically, the highest diversity was detected in the clone library prepared using a combination of primers 27f and 1492r and DNA isolated using the Gram-positive bacteria protocol. The potential of Haliclona sp.-associated bacteria to produce secondary metabolites was studied by cloning and sequencing the polyketide synthase (PKS, type 1) gene using the same DNA samples. Analysis of partial sequences derived from the sponge metagenome revealed 27 unique ketosynthase domains of PKS type I. This study suggests strongly that this Haliclona sp. plays host to diverse novel bacteria with a potential to produce novel polyketides.
Starting Page	199
Ending Page	207
Page Count	9
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	64
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-05-08
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Marine sponge Polyketide synthase Haliclona sp. Bacterial diversity Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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