NDLI: Diversity analysis and characterization of Coleoptera-, Hemiptera- and Nematode-active cry genes in native isolates of Bacillus thuringiensis

Please wait, while we are loading the content...

Characterization of antifungal activity of Paenibacillus ehimensis KWN38 against soilborne phytopathogenic fungi belonging to various taxonomic groups

Methane oxidation and methane driven redox process during sequential reduction of a flooded soil ecosystem

Xylanase and itaconic acid production by Aspergillus terreus NRRL 1960 within a biorefinery concept

Diversity analysis and characterization of Coleoptera-, Hemiptera- and Nematode-active cry genes in native isolates of Bacillus thuringiensis

Genetic diversity of Vicia faba L. and Pisum sativum L. nodulating rhizobia in the central Black Sea region of Turkey

Construction of an RNAi expression vector and transformation into Penicillium chrysogenum

Production and characterization of laccase from Pleurotus ferulae in submerged fermentation

Occurrence of Staphylococcus aureus and evaluation of anti-staphylococcal activity of Lactococcus lactis subsp. lactis in ready-to-eat poultry meat

Expression of modified xynA gene fragments from Bacillus subtilis BE-91

Screening, production, and characterization of dextranase from Catenovulum sp.

Effects of photoperiod, salinity and pH on cell growth and lipid content of Pavlova lutheri

Molecular identification and characterization of clustered regularly interspaced short palindromic repeats (CRISPRs) in Campylobacter lari

PCR-RFLP analysis of fliC, fimH and 16S rRNA genes in Salmonella Typhimurium isolates of varied origin

Analysis of genetic diversity in Phytophthora colocasiae causing leaf blight of taro (Colocasia esculenta) using AFLP and RAPD markers

Diversity of bacteria and polyketide synthase associated with marine sponge Haliclona sp.

Isolation and identification of antimicrobial secondary metabolites from Bacillus cereus associated with a rhabditid entomopathogenic nematode

Butanol production from glycerol by recombinant Escherichia coli

Probiotic-mediated biotransformation of monosodium glutamate to γ-aminobutyric acid: differential production in complex and minimal media and kinetic modelling

Mechanisms of phosphate solubilization by fungal isolates when exposed to different P sources

Production and activity of extracellular lipase from Luteibacter sp.

Azospirillum himalayense sp. nov., a nifH bacterium isolated from Himalayan valley soil, India

Biomass hydrolyzing enzymes from plant pathogen Xanthomonas axonopodis pv. punicae: optimizing production and characterization

Bionectria ochroleuca NOTL33—an endophytic fungus from Nothapodytes foetida producing antimicrobial and free radical scavenging metabolites

Detoxification of enterotoxigenic Bacillus cereus (JX455159) isolated from meat by a local strain of Lactobacillus plantarum (JX282192)

Characterization and identification of phytase-producing bacteria isolated from the gastrointestinal tract of four freshwater teleosts

Assessment of the bacterial community structure in a Brazilian clay soil treated with atrazine

Application of a bacteriocin-like inhibitory substance producing Enterococcus durans E204 strain, isolated from camel milk, to control Listeria monocytogenes CECT 4032 in goat jben

Comparison of bacteriocins production from Enterococcus faecium strains in cheese whey and optimised commercial MRS medium

The strain HEB01 of Fusarium sp., a new pathogen that infects brown soft scale

Production and characterization of a solvent-tolerant protease from a novel marine isolate Bacillus tequilensis P15

The phenotypic, phylogenetic and symbiotic characterization of rhizobia nodulating Lotus sp. in Tunisian arid soils

Erratum to: The phenotypic, phylogenetic and symbiotic characterization of rhizobia nodulating Lotus sp. in Tunisian arid soils

Pelagic nitrification and denitrification rates in an Arctic fjord during early spring

Identification and evaluation of a potential biocontrol agent, Bacillus subtilis, against Fusarium sp. in apple seedlings

Improvement of 5-aminolevulinic acid production by Rubrivivax benzoatilyticus PS-5 with self-flocculation by co-fermentation of precursors and volatile fatty acids under pH-controlled conditions

Degradation of oil tank sludge using long-chain alkane-degrading bacteria

Aspergillus penicillioides—a true halophile existing in hypersaline and polyhaline econiches

A culture-independent PCR-based method for the detection of Lachancea thermotolerans in wine

Identification and nematicidal activity of bacteria isolated from cow dung

Annals of Microbiology : Volume 63

Annals of Microbiology : Volume 62

Annals of Microbiology : Volume 61

Annals of Microbiology : Volume 60

Annals of Microbiology : Volume 59

Annals of Microbiology : Volume 58

Annals of Microbiology : Volume 57

Annals of Microbiology : Volume 56

Diversity analysis and characterization of Coleoptera-, Hemiptera- and Nematode-active cry genes in native isolates of Bacillus thuringiensis

Content Provider	Springer Nature Link
Author	Asokan, Ramasamy Mahadeva Swamy, Hanchipura Mallesh Thimmegowda, Geetha G. Mahmood, Riaz
Copyright Year	2013
Abstract	Applications to combat non-lepidopteran insects are not as common as applications against lepidopteran insects. The aim of the present work was to isolate and identify Bacillus thuringiensis isolates from soil samples using five approaches, viz., analysis of crystal protein production by microscopy; detection of cry gene content by PCR, SDS-PAGE profiling; cloning and sequencing; phylogenetic analysis; and toxicity testing. Two hundred soil samples were used for isolation of B. thuringiensis and a total of 69 putative isolates of B. thuringiensis that produce parasporal crystalline inclusions were isolated from 5,267 Bacillus-like colonies. A bipyramidal inclusion was predominant in 32.2 % of the B. thuringiensis isolates compared to other shapes. Crystal protein profiling of B. thuringiensis isolates by SDS-PAGE analysis showed the presence of bands of 130, 73, 34, 25 and 13 kDa, among which 50–60 kDa bands were present abundantly. PCR analysis revealed the predominance of Coleopteran-active cry genes in these isolates. Variation in nucleotide sequences, crystal morphology and mass of crystal protein(s) purified from the isolates of B. thuringiensis revealed genetic and molecular diversity. Four strains containing Coleopteran-active cry genes showed higher toxicity against Myllocerus undecimpustulatus undatus Marshall (Coleoptera: Curculionidae) adults when compared with B. thuringiensis subsp. morrisoni pathovar tenebrionis. These results are useful in emphasizing the distribution of cry genes and for prognostication of toxicity, and may contribute to the identification of novel candidate genes for bioengineered crop protection.
Starting Page	85
Ending Page	98
Page Count	14
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	64
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-04-30
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Bipyramidal Coleoptera Hemiptera Plant-parasitic nematode Novel cry gene Toxicity Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Diversity of insecticidal crystal protein genes of Bacillus thuringiensis isolated from soil and cloning of novel haplotypes of cry genes

Genetic diversity and functional characterization of endophytic Bacillus thuringiensis isolates from the North Western Indian Himalayas

Characterization of insecticidal crystal proteincry gene ofBacillus thuringiensis from soil of Sichuan Basin, China and cloning of novel haplotypescry gene

Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran

Diversity and Toxicity of Bacillus thuringiensis from Shifting Cultivation (Jhum) Habitat

Suppressing a plant-parasitic nematode with fungivorous behavior by fungal transformation of a Bt cry gene

Molecular characterization of cry/vip genes and efficacy of native bacillus thuringiensis isolates

Esterase electrophoretic polymorphism ofBacillus thuringiensis andBacillus cereus reference strains

Towards novel Cry toxins with enhanced toxicity/broader: a new chimeric Cry4Ba / Cry1Ac toxin

Diversity analysis and characterization of Coleoptera-, Hemiptera- and Nematode-active cry genes in native isolates of Bacillus thuringiensis

Similar Documents

Diversity of insecticidal crystal protein genes of Bacillus thuringiensis isolated from soil and cloning of novel haplotypes of cry genes

Genetic diversity and functional characterization of endophytic Bacillus thuringiensis isolates from the North Western Indian Himalayas

Characterization of insecticidal crystal proteincry gene ofBacillus thuringiensis from soil of Sichuan Basin, China and cloning of novel haplotypescry gene

Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran

Diversity and Toxicity of Bacillus thuringiensis from Shifting Cultivation (Jhum) Habitat

Suppressing a plant-parasitic nematode with fungivorous behavior by fungal transformation of a Bt cry gene

Molecular characterization of cry/vip genes and efficacy of native bacillus thuringiensis isolates

Esterase electrophoretic polymorphism ofBacillus thuringiensis andBacillus cereus reference strains

Towards novel Cry toxins with enhanced toxicity/broader: a new chimeric Cry4Ba / Cry1Ac toxin

Diversity analysis and characterization of Coleoptera-, Hemiptera- and Nematode-active cry genes in native isolates of Bacillus thuringiensis