NDLI: Ribotyping and TCP gene cluster analysis of environmental and clinical Vibrio cholerae strains isolated in Iran

Content Provider	Springer Nature Link
Author	Bakhshi, Bita Mohammadi Barzelighi, Hajar Hosseini Aliabad, Neda Pourshafie, Mohammad R.
Copyright Year	2013
Abstract	The aim of this study was to investigate the presence of TCP gene clusters among clinical and environmental Vibrio cholerae isolates and to explore the genetic relatedness of isolates using ribotyping technique. A total of 50 V. cholerae strains (30 clinical and 20 environmental) were included in this study. Three clinical isolates were negative for TCP cluster genes while the cluster was absent in all of the environmental strains. Ribotyping of rRNA genes with BglI produced 18 different ribotype patterns, three of which belonged to clinical O1 serotype isolates. The remaining 15 ribotypes belonged to clinical non-O1, non-O139 serogroups (two patterns) and environmental non-O1, non-O139 serogroups (13 patterns). Clinical V. cholerae O1 strains from 2004 through 2006 and several environmental non-O1, non-O139 V. cholerae strains from 2006 showed 67.3 % similarity and fell within one single gene cluster. Ribotyping analysis made it possible to further comprehend the close originality of clinical isolates as very little changes have been occurred within rRNA genes of different genotypes of V. cholerae strains through years. In conclusion, ribotyping analysis of environmental V. cholerae isolates showed a substantial genomic diversity supporting the fact that genetic changes within bacterial genome occurs during years in the environment, while only little changes may arise within the genome of clinical isolates.
Starting Page	49
Ending Page	53
Page Count	5
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	64
Issue Number	1
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-03-28
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Ribotyping tcp gene cluster Vibrio cholerae Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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