NDLI: Overexpression of cyclin D1 mRNA in colorectal carcinomas and relationship to clinicopathological features: Anin situ hybridization analysis

Please wait, while we are loading the content...

Detection of TNFα expression in the bone marrow and determination of TNFα production of peripheral blood mononuclear cells in myelodysplastic syndrome

High production of SPARC/osteonectin/BM-40 in mouse metastatic B16 melanoma cell lines

Unique morphological alterations of the HTLV-I transformed C8166 cells by infection with HIV-1

CRP, TNFα, IL-1ra, IL-6, IL-8 and IL-10 in blood serum of colorectal cancer patients

Serum levels of the soluble adhesion molecules in patients with malignant melanoma

A comparative survival evaluation and assessment of interclassification concordance in adult supratentorial astrocytic tumors

Possibilities of preventing osteoradionecrosis during complex therapy of Tumors of the oral cavity

Microvessel count, proliferating cell nuclear antigen and Ki-67 indices in gastric adenocarcinoma

Overexpression of cyclin D1 mRNA in colorectal carcinomas and relationship to clinicopathological features: Anin situ hybridization analysis

Papillary microcarcinoma of the thyroid gland in renal transplant patients : Case reports and review of the literature

Pathology & Oncology Research : Volume 5

Pathology & Oncology Research : Volume 4

Pathology & Oncology Research : Volume 3

Overexpression of cyclin D1 mRNA in colorectal carcinomas and relationship to clinicopathological features: Anin situ hybridization analysis

Content Provider	Springer Nature Link
Author	Kristt, Don Turner, Isaac Koren, Rumelia Ramadan, Edward Gal, Rivka
Copyright Year	2000
Abstract	Increased expression of a key cell cycle regulator, cyclin Dl, may have relevance to carcinogenesis and clinicopathological characteristics of some cancers. This study represents the first application ofin situ hybridization, ISH, to detect cyclin Dl mRNA in tissue sections from colorectal carcinomas. This approach was selected because of its unique potential to clarify whether increased expression of cyclin Dl mRNA correlates with clinical and pathological parameters. The ISH of a non-radioactive oligonucleotide probe (Biogenex) was immunocytochemically detected in paraffin embedded sections from biopsy or resection specimens. Tumors ranged from well to poorly differentiated, and from stages A, B, C, and D. Ten year survival data were available on the majority of patients. Intensity of tumor and background (smooth muscle) signals were independently scored from 0 to 3. Overexpressed cyclin Dl mRNA was seen in 86% of cases compared to back-ground. This frequency is similar to that reported for pancreatic carcinoma. The average signal intensity score in tumor foci was 1.9 with a background score of 0.05 (p < 001). All cases showed specific staining judged by the cytoplasmic localization and a tumor signal:background ratio > 1. Expression did not differentiate cancers based on grade, stage or survival (p>l), but did differentiate carcinoma and severe dysplasia from mild dysplasia. We conclude that ISH of cyclin Dl mRNA is an effective and relatively specific means of detecting activity of this gene in colonic neoplasms. The high frequency of overexpression implies that gene activity by itself is not likely to predict a tumor’s biological or clinical behavior. On the other hand, these data suggest that increased cyclin Dl gene activity may be an early event in colorectal carcinogenesis. They also are consistent with findings showing cyclin Dl is inducible by a variety of oncogene products.
Starting Page	65
Ending Page	70
Page Count	6
File Format	PDF
ISSN	12194956
Journal	Pathology & Oncology Research
Volume Number	6
Issue Number	1
Language	English
Publisher	Springer Netherlands
Publisher Date	2000-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	cyclin Dl in situ hybridization mRNA colon cancer colorectal carcinoma survival grade stage Cancer Research Oncology Pathology Immunology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Prognostic Significance of Cyclin D1 and E-cadherin Expression in Laryngeal Squamous Cell Carcinoma

Clinicopathological Features and Survival of Signet-Ring Cell Carcinoma and Mucinous Adenocarcinoma of Right Colon, Left Colon, and Rectum.

Immunoexpression of cyclin D1 in colorectal carcinomas is not correlated with survival outcome.

TWIST1 Promoter Methylation in Primary Colorectal Carcinoma

Aberrant Expression of Calretinin, D2–40 and Mesothelin in Mucinous and Non-Mucinous Colorectal Carcinomas and Relation to Clinicopathological Features and Prognosis

Analysis of p53 mutation and cyclin d1 expression in breast tumors

The extent of cyclin D1 expression in endometrial pathologies and relevance of cyclin D1 with the clinicopathological features of endometrioid endometrial carcinoma

Strong nuclear EGFR expression in colorectal carcinomas is associated with cyclin-D1 but not with gene EGFR amplification

Association between microvessel density and histologic grade in renal cell carcinomas

Overexpression of cyclin D1 mRNA in colorectal carcinomas and relationship to clinicopathological features: Anin situ hybridization analysis

Similar Documents

Prognostic Significance of Cyclin D1 and E-cadherin Expression in Laryngeal Squamous Cell Carcinoma

Clinicopathological Features and Survival of Signet-Ring Cell Carcinoma and Mucinous Adenocarcinoma of Right Colon, Left Colon, and Rectum.

Immunoexpression of cyclin D1 in colorectal carcinomas is not correlated with survival outcome.

TWIST1 Promoter Methylation in Primary Colorectal Carcinoma

Aberrant Expression of Calretinin, D2–40 and Mesothelin in Mucinous and Non-Mucinous Colorectal Carcinomas and Relation to Clinicopathological Features and Prognosis

Analysis of p53 mutation and cyclin d1 expression in breast tumors

The extent of cyclin D1 expression in endometrial pathologies and relevance of cyclin D1 with the clinicopathological features of endometrioid endometrial carcinoma

Strong nuclear EGFR expression in colorectal carcinomas is associated with cyclin-D1 but not with gene EGFR amplification

Association between microvessel density and histologic grade in renal cell carcinomas

Overexpression of cyclin D1 mRNA in colorectal carcinomas and relationship to clinicopathological features: Anin situ hybridization analysis