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| Content Provider | Springer Nature Link |
|---|---|
| Author | Fan, Hong bo Li, Jun wei Li, Zhi lin Zheng, Wei Tien, Po Guo, De yin |
| Copyright Year | 2007 |
| Abstract | The M and NP genes of H5N1 avian influenza virus (A/chicken/Hubei/489/2004) were amplified by RT-PCR from viral RNA, and cloned into pMD18-T vector respectively. The expression plasmid containing the M gene (pHM6-m) or the NP gene (pHM6-np) was then constructed by inserting the M or NP gene into the pHM6 eukaryote expression vector; the constructed plasmid was then sequenced. 32 BALB/c mice (6-week-old) were divided into four groups at random. Three groups of BALB/c mice were inoculated one time the intramuscular route with either 30 μg of plasmid pHM6-m, 30 μg of plasmid pHM6-np or the mixture of plasmid pHM6-m (15 μg) and pHM6-np(15 μg) respectively. A additional group of mice were injected with 100 μl PBS as controls. Two weeks later, all mice were challenged with homologous H5N1 avian influenza virus, and observed in the following 12 days. The survival rates of mice in the pHM6-m group, the pHM6-np group and mixed plasmids group were 62.5%, 25.0% and 50.0%, respectively. Results showed that effective protection could be provided by either pHM6-m or pHM6-np, but pHM6-m provided a better protective effect than pHM6-np. |
| Starting Page | 46 |
| Ending Page | 52 |
| Page Count | 7 |
| File Format | |
| ISSN | 16740769 |
| Journal | Virologica Sinica |
| Volume Number | 22 |
| Issue Number | 1 |
| e-ISSN | 1995820X |
| Language | English |
| Publisher | SP Wuhan Institute of Virology, CAS |
| Publisher Date | 2009-09-16 |
| Publisher Place | Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | H5N1 influenza virus M gene NP gene Cloning DNA vaccine Microbial Genetics and Genomics Microbiology Biochemistry Oncology Medical Microbiology Virology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Virology Immunology Molecular Medicine |
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