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| Content Provider | Springer Nature Link |
|---|---|
| Author | Lu, Yin ping Wang, Bao ju Dong, Ji hua Liu, Zhao Guan, Shi he Lu, Meng ji Yang, Dong liang |
| Copyright Year | 2007 |
| Abstract | To establish a replication cellular model of hepatitis B virus (HBV) and determine its application in antiviral drug evaluation, we constructed an expression plasmid which contained 1.3 copies of the HBV genome, and measured the level of viral replication after transient transfection in Huh7 cells. We then observed the effect of antiviral drug administration. 1.3 fold of the HBV(ayw) gene fragment was cloned into pCR2.1 by PCR and restriction endonuclease digestion. The recombinant plasmid was transient transfected into Huh7 cells, HBsAg, HBeAg and HBV DNA in supernatant of Huh7 cells were measured by ELISA and real-time PCR respectively; intracellular HBV replicative intermediates and intracellular HBV transcripts were detected by Southern blot and Northern blot respectively. The antiviral effect of adefovir, a novel anti-HBV nucleotide analogue, was evaluated in this cellular model system. The results indicated that a recombinant plasmid of HBV replicon was constructed successfully; the HBV genome carried in plasmid pHBV1.3 could efficiently replicate and be expressed in Huh 7 cells, adefovir could inhibit HBV replication in this cellular model, and the inhibition was dosage-dependent. The conclusion is HBV replicon, which can initiate viral replication efficiently in hepatoma cells, may be a useful tool in the study of HBV replication and antiviral drug. |
| Starting Page | 8 |
| Ending Page | 13 |
| Page Count | 6 |
| File Format | |
| ISSN | 16740769 |
| Journal | Virologica Sinica |
| Volume Number | 22 |
| Issue Number | 1 |
| e-ISSN | 1995820X |
| Language | English |
| Publisher | SP Wuhan Institute of Virology, CAS |
| Publisher Date | 2009-09-16 |
| Publisher Place | Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Hepatitis B virus Infectious replicon Expression vector Microbial Genetics and Genomics Microbiology Biochemistry Oncology Medical Microbiology Virology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Virology Immunology Molecular Medicine |
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