NDLI: Differential induction of apoptosis in human breast cancer cell lines by phenethyl isothiocyanate, a glutathione depleting agent

Content Provider	Springer Nature Link
Author	Syed Alwi, Sharifah S. Cavell, Breeze E. Donlevy, Alison Packham, Graham
Copyright Year	2012
Abstract	Phenethyl isothiocyanate (PEITC) is a naturally occurring electrophile which depletes intracellular glutathione (GSH) levels and triggers accumulation of reactive oxygen species (ROS). PEITC is of considerable interest as a potential chemopreventive/chemotherapeutic agent, and in this work, we have investigated the effects of PEITC on human breast cancer cell lines. Whereas PEITC readily induced apoptosis in MDA-MB-231 cells (associated with rapid activation of caspases 9 and 3, and decreased expression of BAX), MCF7 cells were relatively resistant to the apoptosis promoting effects of PEITC. The relative resistance of MCF7 cells was associated with high basal expression of NRF2, a transcription factor that coordinates cellular protective responses to oxidants and electrophiles and raised intracellular levels of GSH. This raised basal expression of NRF2 appeared to be a response to on-going production of ROS, since treatment with the antioxidant and GSH precursor N-acetylcysteine (NAC) reduced NRF2 expression. Moreover, pre-treatment of MDA-MB-231 cells with NAC rendered these cells relatively resistant to PEITC-induced apoptosis. In summary, our data confirm that PEITC may be an effective chemopreventive/therapeutic agents for breast cancer. However, differences in the basal expression of NRF2 and resultant changes in GSH levels may be an important determinant of sensitivity to PEITC-induced apoptosis.
Starting Page	529
Ending Page	538
Page Count	10
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	17
Issue Number	5
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-02-17
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Phenethyl isothiocyante Breast cancer NRF2 Glutathione Reactive oxygen species Biochemistry Biomedicine general Cancer Research Immunology Cell Biology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

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