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Cell Stress and Chaperones : Volume 22

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Heat shock proteins and survival strategies in congeneric land snails (Sphincterochila) from different habitats

Differential induction of apoptosis in human breast cancer cell lines by phenethyl isothiocyanate, a glutathione depleting agent

Molecular cloning and characterization of Hsp27.6: the first reported small heat shock protein from Apis cerana cerana

Nrf-2 overexpression in mesenchymal stem cells reduces oxidative stress-induced apoptosis and cytotoxicity

Development of stable HSPA1A promoter-driven luciferase reporter HepG2 cells for assessing the toxicity of organic pollutants present in air

Deubiquitinating enzyme BAP1 is involved in the formation and maintenance of the diapause embryos of Artemia

Molecular cloning of heat shock protein 60 (PtHSP60) from Portunus trituberculatus and its expression response to salinity stress

Protein refolding in peroxisomes is dependent upon an HSF1-regulated function

Heat shock proteins induction reduces stress kinases activation, potentially improving insulin signalling in monocytes from obese subjects

Zebrafish HSF4: a novel protein that shares features of both HSF1 and HSF4 of mammals

The heat shock response in congeneric land snails (Sphincterochila) from different habitats

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Development of stable HSPA1A promoter-driven luciferase reporter HepG2 cells for assessing the toxicity of organic pollutants present in air

Content Provider	Springer Nature Link
Author	Xin, Lili Li, Xiaohai Deng, Huaxin Kuang, Dan Dai, Xiayun Huang, Suli Wang, Feng He, Meian Currie, R. William Wu, Tangchun
Copyright Year	2012
Abstract	HSPA1A (HSP70-1) is a highly inducible heat shock gene up-regulated in response to environmental stresses and pollutants. The aim of our study was to evaluate the sensitivity of the stable metabolically competent HepG2 cells containing a human HSPA1A promoter-driven luciferase reporter (HepG2-luciferase cells) for assessing the toxicity of organic pollutants present in air. The HepG2-luciferase cells were validated by heat shock treatment and testing three organic compounds (pyrene, benzo[a]pyrene, and formaldehyde) that are ubiquitous in the air. The maximal level of HSPA1A (HSP70-1) and relative luciferase activity induced by heat shock were over three and nine times the control level, respectively. Pyrene, benzo[a]pyrene, and formaldehyde all induced significantly elevated levels of relative luciferase activity in a dose-dependent manner. Extractable organic matter (EOM) from urban traffic and coke oven emissions in ambient air were tested on the HepG2-luciferase cells. The traffic EOM induced significant increase in relative luciferase activity at concentrations of picogram per liter. The coke oven EOM produced a strong dose-dependent induction of relative luciferase activity up to six times the control value. Significant increases in relative luciferase activity were observed at concentrations that were as low, or lower than the concentrations that the tested organic pollutants decreased cell viability, and increased malondialdehyde concentration, Olive tail moment, and micronuclei frequency. Therefore, we conclude that the HepG2-luciferase cells are a valuable tool for rapid screening of the overall toxicity of organic pollutants present in air.
Starting Page	567
Ending Page	576
Page Count	10
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	17
Issue Number	5
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-02-26
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	HSPA1A promoter activity Luciferase reporter Organic pollutants Cell viability Cellular damage Toxicity assessment Biochemistry Biomedicine general Cancer Research Immunology Cell Biology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

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Development of stable HSPA1A promoter-driven luciferase reporter HepG2 cells for assessing the toxicity of organic pollutants present in air.

Development of stable HSPA1A promoter-driven luciferase reporter HepG2 cells for assessing the toxicity of organic pollutants present in air

Development of rapid and highly sensitive HSPA1A promoter-driven luciferase reporter system for assessing oxidative stress associated with low-dose photodynamic therapy

Development of rapid and highly sensitive HSPA1A promoter-driven luciferase reporter system for assessing oxidative stress associated with low-dose photodynamic therapy.

Comparative oxidative stress elicited by nanosilver in stable HSPA1A promoter-driven luciferase reporter HepG2 and A549 cells

Development of rapid and highly sensitive HSPA1A promoter-driven luciferase reporter system for assessing oxidative stress associated with low-dose photodynamic therapy

Development of a multifunctional luciferase reporters system for assessing endoplasmic reticulum-targeting photosensitive compounds

An artificial HSE promoter for efficient and selective detection of heat shock pathway activity

Stress-induced localization of HSPA6 (HSP70B′) and HSPA1A (HSP70-1) proteins to centrioles in human neuronal cells

Development of stable HSPA1A promoter-driven luciferase reporter HepG2 cells for assessing the toxicity of organic pollutants present in air

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Development of stable HSPA1A promoter-driven luciferase reporter HepG2 cells for assessing the toxicity of organic pollutants present in air.

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Development of rapid and highly sensitive HSPA1A promoter-driven luciferase reporter system for assessing oxidative stress associated with low-dose photodynamic therapy

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Development of stable HSPA1A promoter-driven luciferase reporter HepG2 cells for assessing the toxicity of organic pollutants present in air