NDLI: Molecular cloning and characterization of Hsp27.6: the first reported small heat shock protein from Apis cerana cerana

Content Provider	Springer Nature Link
Author	Liu, Zhaohua Xi, Dongmei Kang, Mingjiang Guo, Xingqi Xu, Baohua
Copyright Year	2012
Abstract	Small heat shock proteins (sHSPs) play an important role in the cellular defense of prokaryotic and eukaryotic organisms against a variety of internal and external stressors. In this study, a cDNA clone encoding a member of the α-crystallin/sHSP family, termed AccHsp27.6, was isolated from Apis cerana cerana. The full-length cDNA is 1,014 bp in length and contains a 708-bp open reading frame encoding a protein of 236 amino acids with a calculated molecular weight of 27.6 kDa and an isoelectric point of 7.53. Seven putative heat shock elements and three NF-κB binding sites were present in the 5′-flanking region, suggesting a possible function in immunity. A semi-quantitative RT-PCR analysis indicated that AccHsp27.6 was expressed in all tested tissues and at different developmental stages. Furthermore, expression of the AccHsp27.6 transcript was induced by exposure to heat shock, H$_{2}$O$_{2}$, a number of different chemicals (including SO$_{2}$, formaldehyde, alcohol, acetone, chloroform, and the pesticides phoxime and acetamiprid), and the microbes Staphylococcus aureus and Micrococcus luteus. In contrast, the mRNA expression could be repressed by CO$_{2}$, the pesticides pyriproxyfen and cyhalothrin, and the microbes Bacillus subtilis and Pseudomonas aeruginosa. Notably, the recombinant AccHsp27.6 protein exhibited significant in vitro molecular chaperone activity and antimicrobial activity. Taken together, these results suggest that AccHsp27.6 might play an important role in the response to abiotic and biotic stresses and in immune reactions.
Starting Page	539
Ending Page	551
Page Count	13
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	17
Issue Number	5
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-02-19
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Apis cerana cerana Expression Gene cloning Immunological function Small heat shock proteins Biochemistry Biomedicine general Cancer Research Immunology Cell Biology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

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