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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ferri, D. V. Munhoz, C. F. Neves, P. M. O. Ferracin, L. M. Sartori, D. Vieira, M. L. C. Fungaro, M. H. P. |
| Copyright Year | 2012 |
| Abstract | The banana weevil Cosmopolites sordidus (Germar) is one of a number of pests that attack banana crops. The use of the entomopathogenic fungus Beauveria bassiana as a biological control agent for this pest may contribute towards reducing the application of chemical insecticides on banana crops. In this study, the genetic variability of a collection of Brazilian isolates of B. bassiana was evaluated. Samples were obtained from various geographic regions of Brazil, and from different hosts of the Curculionidae family. Based on the DNA fingerprints generated by RAPD and AFLP, we found that 92 and 88 % of the loci were polymorphic, respectively. The B. bassiana isolates were attributed to two genotypic clusters based on the RAPD data, and to three genotypic clusters, when analyzed with AFLP. The nucleotide sequences of nuclear ribosomal DNA intergenic spacers confirmed that all isolates are in fact B. bassiana. Analysis of molecular variance showed that variability among the isolates was not correlated with geographic origin or hosts. A RAPD-specific marker for isolate CG 1024, which is highly virulent to C. sordidus, was cloned and sequenced. Based on the sequences obtained, specific PCR primers BbasCG1024F (5′-TGC GGC TGA GGA GGA CT-3′) and BbasCG1024R (5′-TGC GGC TGA GTG TAG AAC-3′) were designed for detecting and monitoring this isolate in the field. |
| Starting Page | 569 |
| Ending Page | 574 |
| Page Count | 6 |
| File Format | |
| ISSN | 00468991 |
| Journal | Indian Journal of Microbiology |
| Volume Number | 52 |
| Issue Number | 4 |
| e-ISSN | 09737715 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-08-01 |
| Publisher Institution | Association of Microbiologists of India |
| Publisher Place | India |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | RAPD AFLP Strain-specific molecular marker Environmental monitoring Biological control Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
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