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| Content Provider | Springer Nature Link |
|---|---|
| Author | Sharma, Nandita Tanksale, Himgouri Kapley, Atya Purohit, Hemant J. |
| Copyright Year | 2012 |
| Abstract | Metagenomic libraries herald the era of magnifying the microbial world, tapping into the vast metabolic potential of uncultivated microbes, and enhancing the rate of discovery of novel genes and pathways. In this paper, we describe a method that facilitates the extraction of metagenomic DNA from activated sludge of an industrial wastewater treatment plant and its use in mining the metagenome via library construction. The efficiency of this method was demonstrated by the large representation of the bacterial genome in the constructed metagenomic libraries and by the functional clones obtained. The BAC library represented 95.6 times the bacterial genome, while, the pUC library represented 41.7 times the bacterial genome. Twelve clones in the BAC library demonstrated lipolytic activity, while four clones demonstrated dioxygenase activity. Four clones in pUC library tested positive for cellulase activity. This method, using FTA cards, not only can be used for library construction, but can also store the metagenome at room temperature. |
| Starting Page | 538 |
| Ending Page | 543 |
| Page Count | 6 |
| File Format | |
| ISSN | 00468991 |
| Journal | Indian Journal of Microbiology |
| Volume Number | 52 |
| Issue Number | 4 |
| e-ISSN | 09737715 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-03-25 |
| Publisher Institution | Association of Microbiologists of India |
| Publisher Place | India |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Activated biomass Cellulase FTA elute micro card Lipase Metagenomic library Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
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