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| Content Provider | Springer Nature Link |
|---|---|
| Author | Fu, Guanhua Wang, Yongtao Wang, Dandan Zhou, Chenyan |
| Copyright Year | 2012 |
| Abstract | A xylanase gene (xynZF-2) from the Aspergillus niger XZ-3S was cloned and expressed in Escherichia coli. The coding region of the gene was separated by only one intron with the 68 bp in length. It encoded 225 amino acid residues of a protein with a calculated molecular weight of 24.04 kDa plus a signal peptide of 18 amino acids. The amino acid sequence of the xynZF-2 gene had a high similarity with those of family 11 of glycosyl hydrolases reported from other microorganisms. The mature peptide encoding cDNA was subcloned into pET-28a(+) expression vector. The resultant recombinant plasmid pET-28a-xynZF-2 was transformed into E. coli BL21(DE3), and finally the recombinant strain BL21/xynZF-2 was obtained. A maximum activity of 42.33 U/mg was gained from cellular of E. coli BL21/xynZF-2 induced by IPTG. The optimum temperature and pH for recombinant enzyme which has a good stability in alkaline conditions were 40 °C and 5.0, respectively. Fe$^{3+}$ had an active effect on the enzyme obviously. |
| Starting Page | 682 |
| Ending Page | 688 |
| Page Count | 7 |
| File Format | |
| ISSN | 00468991 |
| Journal | Indian Journal of Microbiology |
| Volume Number | 52 |
| Issue Number | 4 |
| e-ISSN | 09737715 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-10-10 |
| Publisher Institution | Association of Microbiologists of India |
| Publisher Place | India |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aspergillus niger Xylanase Cloning Expression Enzymatic properties Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
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