NDLI: Molecular Analysis of Cocaine-Induced Endothelial Dysfunction: Role of Endothelin-1 and Nitric Oxide

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Molecular Analysis of Cocaine-Induced Endothelial Dysfunction: Role of Endothelin-1 and Nitric Oxide

Content Provider	Springer Nature Link
Author	Pradhan, Leena Mondal, Debasis Chandra, Surabhi Ali, Mussa Agrawal, Krishna C.
Copyright Year	2008
Abstract	Cocaine remains the most frequently used illicit substance. Although cocaine-induced atherosclerosis is well documented, its mechanism of action on human vascular endothelial cells has not been determined. Nitric oxide (NO) and endothelin-1 (ET-1) are involved in endothelial cell activation and leukocyte recruitment. The present study monitored the effects of cocaine on NO and ET-1 production in human aortic endothelial cells (HAECs) and the effects of sodium nitroprusside (SNP) and BQ-123 on leukocyte adhesion to HAECs. Acute exposure to cocaine (1 and 3 μM) significantly increased ET-1 production (2-fold) and ET-1 receptor type-A (ET$_{A}$R) protein expression, within 6–12 h. Cocaine exposure for a longer duration (24–72 h) showed a temporal decrease in both NO production and endothelial NO-synthase (eNOS) expression. The cocaine-mediated suppression of NO was ameliorated by co-treatment of cells with the ET$_{A}$R blocker, BQ-123 (5 μM). Furthermore, both short-term (24 h) and long-term (72 h) exposure to cocaine increased endothelial adhesion of monocytes (U937 cells) by 20% and 40%, respectively, which were also suppressed by BQ-123 and SNP co-treatment. These data suggest that a concomitant increase in both ET-1 and ET$_{A}$R expression in cocaine exposed HAECs may enhance signaling via the ET$_{A}$R which decreases eNOS expression and NO production, and ultimately results in endothelial activation and leukocyte adhesion. Our findings implicate a molecular mechanism of action of cocaine and a therapeutic effect of ET$_{A}$R-specific inhibitor in suppressing the cocaine-induced endothelial dysfunction.
Starting Page	161
Ending Page	171
Page Count	11
File Format	PDF
ISSN	15307905
Journal	Cardiovascular Toxicology
Volume Number	8
Issue Number	4
e-ISSN	15590259
Language	English
Publisher	Humana Press Inc
Publisher Date	2008-09-24
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cocaine Endothelial dysfunction HAECs Leukocyte adhesion Endothelin-1 Endothelin receptors Nitric oxide Cardiology Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Toxicology Cardiology and Cardiovascular Medicine

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