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| Content Provider | Springer Nature Link |
|---|---|
| Author | Song, Kedong Li, Wenfang Wang, Hong Wang, Hai Liu, Tianqing Ning, Ruiming Wang, Ling |
| Copyright Year | 2012 |
| Abstract | The purpose of this study was to evaluate the differentiation potential of human adipose-derived stem cells (hADSCs) into adipocytes by coculturing them with human mature adipocytes. The transwell culture system was utilized for indirect coculture of hADSCs and human mature adipocytes at four different hADSCs-to-mature adipocytes ratios, i.e., 1:5, 1:1, 2:1, and 5:1. After 8 days of coculture, the Oil Red O and Trypan Blue stainings were performed for the evaluation of adipogenic differentiation of hADSCs. In addition, flow cytometric analysis and Hoechst 33342/PI double staining were performed after 20 days of coculture. The Oil Red O and Trypan Blue stainings showed that hADSCs with high viability could not differentiate into mature adipocytes after 8 or 20 days of coculture. However, flow cytometric analysis indicated that CD105 expression of hADSCs decreased after 20 days of coculture. These results indicated that hADSCs cocultured with human adult adipocytes could not successfully differentiate into adipocytes. |
| Starting Page | 2381 |
| Ending Page | 2387 |
| Page Count | 7 |
| File Format | |
| ISSN | 02732289 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume Number | 167 |
| Issue Number | 8 |
| e-ISSN | 15590291 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-06-21 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Adipocytes Adipose-derived stem cells Coculture Adipogenic differentiation Oil Red O staining Biochemistry Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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