NDLI: Oil red-O stains non-adipogenic cells: a precautionary note

Content Provider	Springer Nature Link
Author	Kinkel, Adrienne D. Fernyhough, Melinda E. Helterline, Deri L. Vierck, Janet L. Oberg, Karen S. Vance, Tyler J. Hausman, Gary J. Hill, Rodney A. Dodson, Michael V.
Copyright Year	2005
Abstract	Bovine adipofibroblasts, 3T3-L1 cells, L-6 myogenic cells, and sheep satellite cells were allowed to proliferate for 48 h. Oil red-O (ORO) was dissolved in three different solvents isopropanol, propylene glycol and triethyl phosphate. At 48 h, the proliferative cultures were stained with the three stains. ORO stain prepared in both propylene glycol and triethyl phosphate resulted in bright red droplets appearing in all cultures, whereas ORO dissolved in isopropanol was not taken up by any of the cells. These data suggest that certain preparations of ORO may stain cells in non-adipogenic lineages as well as undifferentiated pre-adipocytes. Caution must be exercised when choosing solvents for ORO in differentiation studies using cells of the fat/adipose lineage.
Starting Page	49
Ending Page	56
Page Count	8
File Format	PDF
ISSN	09209069
Journal	Cytotechnology
Volume Number	46
Issue Number	1
e-ISSN	15730778
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-06-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adipocyte Adipofibroblasts Cell culture Oil red-O Satellite cell Staining Biomedicine general Biotechnology Biochemistry Proteomics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Clinical Biochemistry Bioengineering Biomedical Engineering Biotechnology

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Oil red-O stains non-adipogenic cells: a precautionary note.

Oil red-O stains non-adipogenic cells: a precautionary note

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