NDLI: Detection and Clinical Significance of CD44v6 and Integrin-β1 in Pancreatic Cancer Patients using a Triplex Real-Time RT-PCR Assay

Content Provider	Springer Nature Link
Author	Zhou, Gang Chiu, David Qin, Dajiang Niu, Lizhi Cai, Jinlei He, Lihua Huang, Wenhao Xu, Kecheng
Copyright Year	2012
Abstract	The cell adhesion molecules CD44v6 and integrin-β1 are associated with the progression and metastasis of cancer. A novel triplex real-time reverse transcription polymerase chain reaction (qRT-PCR) assay was developed to quantify CD44v6 and integrin-β1 gene expression in peripheral blood mononuclear cells from 30 pancreatic cancer (PC) patients and 12 healthy individuals. The standard curve of the triplex qRT-PCR was constructed by optimizing the reaction condition and the amplification efficiency was 102.5, 101.1, and 100.6 % for CD44v6, integrin-β1 and endogenous gene (β-actin) amplification. Nonspecific bands were not observed from the triplex qRT-PCR amplification and the detection limit of this assay was 100 copies. Expression levels of CD44v6 and integrin-β1 gene were significantly lower in healthy individuals than PC patients (P < 0.05). CD44v6 and integrin-β1 gene expression were not associated with the sex, age, and tumor position in PC (P > 0.05). CD44v6 gene expression was significantly associated with clinical stage, liver metastasis, and tumor size (P < 0.05). Integrin-β1 gene expression was significantly associated with clinical stage and liver metastasis (P < 0.05). This triplex qRT-PCR assay may provide a useful tool for diagnosis, prognosis, and therapeutic evaluation in PC.
Starting Page	2257
Ending Page	2268
Page Count	12
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	167
Issue Number	8
e-ISSN	15590291
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-06-14
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	CD44v6 Integrin-β1 Quantitative Real-time RT-PCR Pancreatic cancer Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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