NDLI: Nonviral regenerative gene therapy of mammalian cutaneous damages

Please wait, while we are loading the content...

Cell and Tissue Biology : Volume 11

Cell and Tissue Biology : Volume 10

Cell and Tissue Biology : Volume 9

Cell and Tissue Biology : Volume 8

Cell and Tissue Biology : Volume 7

Cell and Tissue Biology : Volume 6

Cell and Tissue Biology : Volume 5

Cell and Tissue Biology : Volume 4

Cell and Tissue Biology : Volume 4, Issue 6, December 2010

Cell and Tissue Biology : Volume 4, Issue 5, October 2010

Cell and Tissue Biology : Volume 4, Issue 4, August 2010

Neurogenic differentiation of mesenchymal stem cells: Transgenic approach

Influence of primary adhesive interactions with fibronectin on clonal growth and osteogenic potential of rat mesenchymal stromal cells

CD14$^{++}$CD16$^{−}$ and CD14$^{+}$CD16$^{+}$ human monocyte adhesion to endothelial cells

Change in distribution of mobile genetic elements in genome of Drosophila melanogaster: Cause or consequence of selection for quantitative traits?

Doxorubicin and menadione decrease cell proliferation of Saccharomyces cerevisiae by different mechanisms

Nuclear matrix organization of chromocenters in cultured murine fibroblasts

Localization and composition of renal immunodeposits in mice developing HgCl$_{2}$-induced autoimmune process

Comparative analysis of SGLT1 and GLUT2 transporters distribution in rat small-intestine enterocytes and Caco-2 cells during hexose absorption

Nonviral regenerative gene therapy of mammalian cutaneous damages

Metalloprotease-mediated HB-EGF release regulates EGF receptor transactivation in A431 cells at oxidative stress

GABA- and glycine-immunoreactive synapses in spinal cord of frog Rana temporaria

Optimization of dopaminergic neuron counting in substantia nigra of parkinsonian mice

Effects of inhibitors of serine/threonine protein kinases on Arabidopsis thaliana root morphology and microtubule organization in its cells

Cell and Tissue Biology : Volume 4, Issue 3, June 2010

Cell and Tissue Biology : Volume 4, Issue 2, April 2010

Cell and Tissue Biology : Volume 4, Issue 1, February 2010

Cell and Tissue Biology : Volume 3

Cell and Tissue Biology : Volume 2

Cell and Tissue Biology : Volume 1

Nonviral regenerative gene therapy of mammalian cutaneous damages

Content Provider	Springer Nature Link
Author	Efremov, A. M. Dukhovliv, I. V. Dizhe, E. B. Burov, S. V. Leko, M. V. Akifiev, B. N. Mogilenko, D. A. Ivav, I. A. Perevozchikov, A. P. Orlov, S. V.
Copyright Year	2010
Abstract	The rate and character of superficial tissue regeneration after wounds, burns, and other traumas depend on cell proliferation within the damaged area. The acceleration of wound healing via the stimulation of cell proliferation and extracellular matrix synthesis is one of the most important tasks of modern medicine. There are gene therapy approaches to wound treatment, such as the transfer of genes that encode mitogenic growth factors to the wound area. The most important step in the development of the gene therapy approaches is the design of gene delivery tools. Despite the high efficiency of viral vectors, the nonviral approaches have some advantages (low toxicity, low immunogenity, safety, and the absence of side effects). Among the nonviral gene delivery tools molecular conjugates are the most popular due to their efficiency, simplicity, and the capacity for targeted gene transfer. In the present work, we have developed two molecular conjugates, NLS-TSF7 and NLS-TSF12, which consist of the modified signal of the nuclear localization of the T-antigen of the SV40 virus (cationic part) and the peptide ligands of the mammalian transferrin receptor (ligand part). Those conjugates bind to plasmid DNA via the formation of polyelectrolytic complexes and are able to deliver plasmid DNA into cells that express transferrin receptors through receptor-mediated endocytosis. The transfer of the expression vector of the luciferase gene in the complex with the molecular conjugate NLS-TSF7 to murine surface tissues led to about the 100-fold increase of luciferase activity in comparison with the transfer of the free expression vector. The treatment of mice with incised wounds with complexes of the expression vector of the synthetic human gene that encodes insulin-like growth factor 1 with molecular conjugate NLS-TSF7 led to the acceleration of wound healing in comparison with mice treated with the free expression vector. The obtained results confirm the high efficiency of the developed approach to regenerative gene therapy for treating the superficial tissue damage of mammals.
Starting Page	362
Ending Page	371
Page Count	10
File Format	PDF
ISSN	1990519X
Journal	Cell and Tissue Biology
Volume Number	4
Issue Number	4
e-ISSN	19905203
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2010-09-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	molecular conjugates gene transfer regenerative gene therapy transferrin receptor insulin-like growth factor 1 Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Insulin-like growth factor-2 regulates basal retinal insulin receptor activity.

Endothelial Cell Mediated Promotion of Ciliated Cell Differentiation of Human Airway Basal Cells via Insulin and Insulin-Like Growth Factor 1 Receptor Mediated Signaling

Sex differences and left–right asymmetries in expression of insulin and insulin-like growth factor-1 receptors in developing rat hippocampus

Expression and purification of functional insulin and insulin-like growth factor 1 holoreceptors from mammalian cells

Genetic Analysis of Type-1 Insulin-like Growth Factor Receptor Signaling through Insulin Receptor Substrate-1 and -2 in Pancreatic β Cells

Lentivirus-mediated RNAi knockdown of insulin-like growth factor-1 receptor inhibits growth, reduces invasion, and enhances radiosensitivity in human osteosarcoma cells

The effects of maternal diabetes on expression of insulin-like growth factor-1 and insulin receptors in male developing rat hippocampus

How insulin-like growth factor I binds to a hybrid insulin receptor type 1 insulin-like growth factor receptor.

Lentivirus-mediated shRNA targeting insulin-like growth factor-1 receptor (IGF-1R) enhances chemosensitivity of osteosarcoma cells in vitro and in vivo

Nonviral regenerative gene therapy of mammalian cutaneous damages

Similar Documents

Insulin-like growth factor-2 regulates basal retinal insulin receptor activity.

Endothelial Cell Mediated Promotion of Ciliated Cell Differentiation of Human Airway Basal Cells via Insulin and Insulin-Like Growth Factor 1 Receptor Mediated Signaling

Sex differences and left–right asymmetries in expression of insulin and insulin-like growth factor-1 receptors in developing rat hippocampus

Expression and purification of functional insulin and insulin-like growth factor 1 holoreceptors from mammalian cells

Genetic Analysis of Type-1 Insulin-like Growth Factor Receptor Signaling through Insulin Receptor Substrate-1 and -2 in Pancreatic β Cells

Lentivirus-mediated RNAi knockdown of insulin-like growth factor-1 receptor inhibits growth, reduces invasion, and enhances radiosensitivity in human osteosarcoma cells

The effects of maternal diabetes on expression of insulin-like growth factor-1 and insulin receptors in male developing rat hippocampus

How insulin-like growth factor I binds to a hybrid insulin receptor type 1 insulin-like growth factor receptor.

Lentivirus-mediated shRNA targeting insulin-like growth factor-1 receptor (IGF-1R) enhances chemosensitivity of osteosarcoma cells in vitro and in vivo

Nonviral regenerative gene therapy of mammalian cutaneous damages