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| Content Provider | Springer Nature Link |
|---|---|
| Author | Oh, Myung Jin Na, Hye Ryun Choi, Hong Keun Liu, Jang Ryol Kim, Suk Weon |
| Copyright Year | 2010 |
| Abstract | Culture conditions were established for high frequency plant regeneration via somatic embryogenesis from cell suspension cultures of Nymphoides coreana. Zygotic embryos formed pale-yellow globular structures and calluses at a frequency of 85.6% when cultured on half-strength Murashige and Skoog (MS) medium supplemented with 0.3 mg l$^{−1}$ of 2,4-D. However, the frequency of pale-yellow globular structures and white callus formation decreased slightly with an increasing concentration of 2,4-D up to 10 mg l$^{−1}$ with the frequency rate falling to 16.7%. Cell suspension cultures were established from zygotic embryo-derived calluses using half-strength MS medium supplemented with 0.3 mg l$^{−1}$ of 2,4-D. Upon plating onto half-strength MS basal medium, over 92.3% of cell aggregates gave rise to numerous somatic embryos and developed into plantlets. Regenerated plantlets were successfully transplanted into potting soil and achieved full growth to an adult plant in a growth chamber. The high frequency plant regeneration system for Nymphoides coreana established in this study will be useful for genetic manipulation and cryopreservation of this species. |
| Starting Page | 125 |
| Ending Page | 128 |
| Page Count | 4 |
| File Format | |
| ISSN | 18635466 |
| Journal | Plant Biotechnology Reports |
| Volume Number | 4 |
| Issue Number | 2 |
| e-ISSN | 18635474 |
| Language | English |
| Publisher | Springer Japan |
| Publisher Date | 2010-02-05 |
| Publisher Place | Japan |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Nymphoides coreana Plant regeneration Somatic embryogenesis Zygotic embryo culture Agriculture Biotechnology Plant Biochemistry Cell Biology Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Biotechnology |
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